A phosphate starvation response-regulated receptor-like kinase, OsADK1, is required for mycorrhizal symbiosis and phosphate starvation responses

doi:10.1111/nph.18546

	A phosphate starvation response-regulated receptor-like kinase, OsADK1, is required for mycorrhizal symbiosis and phosphate starvation responses
	Shi, Jincai 1,2; Zhao, Boyu 1,2; Jin, Rui 2; Hou, Ling 2,3; Zhang, Xiaowei 2; Dai, Huiling 2; Yu, Nan 1; Wang, Ertao2,4
	2022-11
发表期刊	NEW PHYTOLOGIST (IF:8.3[JCR-2023],10.2[5-Year])
ISSN	0028-646X
EISSN	1469-8137
卷号	236 期号:6 页码:2282-2293
发表状态	已发表
DOI	10.1111/nph.18546
摘要	Most land plants associate with arbuscular mycorrhizal (AM) fungi to secure mineral nutrient acquisition, especially that of phosphorus. A phosphate starvation response (PHR)-centered network regulates AM symbiosis. Here, we identified 520 direct target genes for the rice transcription factor OsPHR1/2/3 during AM symbiosis using transcriptome deep sequencing and DNA affinity purification sequencing. These genes were involved in strigolactone biosynthesis, transcriptional reprogramming, and bidirectional nutrient exchange. Moreover, we identified the receptor-like kinase, Arbuscule Development Kinase 1 (OsADK1), as a new target of OsPHR1/2/3. Electrophoretic mobility shift assays and transactivation assays showed that OsPHR2 can bind directly to the P1BS elements within the OsADK1 promoter to activate its transcription. OsADK1 appeared to be required for mycorrhizal colonization and arbuscule development. In addition, hydroponic experiments suggested that OsADK1 may be involved in plant Pi starvation responses. Our findings validate a role for OsPHR1/2/3 as master regulators of mycorrhizal-related genes involved in various stages of symbiosis, and uncover a new RLK involved in AM symbiosis and plant Pi starvation responses.
关键词	arbuscular mycorrhizal symbiosis arbuscule development DAP-seq OsADK1 OsPHR1 2 3 receptor-like kinase rice (Oryza sativa)
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Science Foundation["32088102","31730103","31825003","32050081","31870218"] ; CAS Project for Young Scientists in Basic Research[YSBR-011] ; Strategic Priority Research Program 'Molecular Mechanism of Plant Growth and Development' of the Chinese Academy of Sciences[XDB27040207] ; National Key R&D Program of China["2019YFA0904703","2016YFA0500502"]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000880741500001
出版者	WILEY
Scopus 记录号	2-s2.0-85141930156
来源库	Scopus
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/248879
专题	生命科学与技术学院_特聘教授组_王二涛组
通讯作者	Yu, Nan; Wang, Ertao
作者单位	1.Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai,200234,China 2.National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,SIBS,Chinese Academy of Sciences,Shanghai,200032,China 3.University of Chinese Academy of Sciences,Beijing,100049,China 4.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Shi, Jincai,Zhao, Boyu,Jin, Rui,et al. A phosphate starvation response-regulated receptor-like kinase, OsADK1, is required for mycorrhizal symbiosis and phosphate starvation responses[J]. NEW PHYTOLOGIST,2022,236(6):2282-2293.
APA	Shi, Jincai.,Zhao, Boyu.,Jin, Rui.,Hou, Ling.,Zhang, Xiaowei.,...&Wang, Ertao.(2022).A phosphate starvation response-regulated receptor-like kinase, OsADK1, is required for mycorrhizal symbiosis and phosphate starvation responses.NEW PHYTOLOGIST,236(6),2282-2293.
MLA	Shi, Jincai,et al."A phosphate starvation response-regulated receptor-like kinase, OsADK1, is required for mycorrhizal symbiosis and phosphate starvation responses".NEW PHYTOLOGIST 236.6(2022):2282-2293.