Using Pooled miR30-shRNA Library for Cancer Lethal and Synthetic Lethal Screens

doi:10.1007/978-1-4939-0992-6_5

	Using Pooled miR30-shRNA Library for Cancer Lethal and Synthetic Lethal Screens
	2014
	Lee, Liam Changwoo 1; Gao, Shaojian 1; Li, Qiuning2 ; Luo, Ji 1
来源专著	Methods in Molecular Biology
出版地	999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA
出版者	HUMANA PRESS INC
ISSN	1064-3745
卷号	1176
页码	45-58
摘要	Pooled shRNA library is a powerful, rapid, and cost-effective technology to carry out functional genomic screens in mammalian cells. This approach has been applied extensively to identify genetic dependencies in cancer cells that might be exploited for therapeutic purposes. In this chapter we provide a detailed protocol for using the Hannon-Elledge miR30-based library to conduct dropout screens in cancer cell lines. This protocol is readily adaptable to other pooled shRNA libraries and should facilitate the functional annotation of the human genome.
关键词	RNAi Synthetic lethality Cancer cells miR30-based library Dropout screen
DOI	10.1007/978-1-4939-0992-6_5
收录类别	BSCI
语种	英语
WOS记录号	WOS:000339721100006
通讯作者	Lee, Liam Changwoo
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Genetics & Heredity ; Pathology
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity ; Pathology
原始文献类型	Article ; Book Chapter
引用统计	正在获取...
文献类型	专著章节
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2445
专题	生命科学与技术学院_行政办公室生命科学与技术学院
通讯作者	Lee, Liam Changwoo
作者单位	1.NCI, Med Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Lee, Liam Changwoo,Gao, Shaojian,Li, Qiuning,et al. Using Pooled miR30-shRNA Library for Cancer Lethal and Synthetic Lethal Screens. 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA:HUMANA PRESS INC,2014:45-58.