Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks

doi:10.3390/su141912593

	Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks
	Chen, Xiaojia1,2,3 ; Wu, Chengjian 4; Li, Qinyu1,2,3 ; Zhou, Peng 5; Chen, Zheng 5; Han, Yang 6; Shi, Jiping1,2,3 ; Zhao, Zhijun 1,3
	2022-10
发表期刊	SUSTAINABILITY
EISSN	2071-1050
卷号	14 期号:19
DOI	10.3390/su141912593
摘要	Antibiotic resistance caused by antibiotic resistance genes (ARGs) threatens human health. ARGs in animal manure can be degraded by composting. This study explored the changes in ARGs and microbial communities during co-composting of pig manure and tea stalks with (T) thermophilic microbial agents, including Geobacillus toebii ZF1 and Geobacillus sp. ZF2 for 15 days in a 4 L vacuum flask. Composting without thermophilic microbial agents served as control (CK). The results showed that the compost temperature of group T peaked at 72.1 degrees C and maintained above 70 degrees C for 4 days. The maximum temperature of group CK was 64.0 degrees C. The seed germination index showed that group T had reached maturity on day 9, while group CK reached maturity on day 15. The dominant bacteria in group CK were Bacillus. In group T, the dominant bacteria changed from Bacillus and Corynebacterium to Geobacillus. Compared with that of group CK, group T increased the removal of cmx, baeS and TaeA by 24.6%, 18.6% and 12.1%, respectively. Group T promoted and inhibited the removal of arlR and novA, respectively (p < 0.05), while group CK showed the opposite effect. Network analysis suggested that group T effectively inactivated Dietzia, Clostridium and Corynebacterium (p < 0.05) and promoted the removal of cmx and baeS. These results showed that thermophilic microbial agents could accelerate the maturation of pig manure and tea stalks, change microbial communities and promote the removal of ARGs. It is of great significance to reduce the spread of ARGs and, in turn, human health risks.
关键词	antibiotic resistance genes thermophilic agents CARD pig manure tea stalks microbial communities
URL	查看原文
收录类别	SCIE ; SSCI
语种	英语
WOS研究方向	Science & Technology - Other Topics ; Environmental Sciences & Ecology
WOS类目	Green & Sustainable Science & Technology ; Environmental Sciences ; Environmental Studies
WOS记录号	WOS:000867982600001
出版者	MDPI
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/243289
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_史吉平组
通讯作者	Shi, Jiping; Zhao, Zhijun
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, Shanghai 201210, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Fuzhou Kaijie Foodstuff Dev Co Ltd, Fuzhou 350003, Peoples R China; 5.Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Shanghai 200093, Peoples R China; 6.Northeast Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Chen, Xiaojia,Wu, Chengjian,Li, Qinyu,et al. Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks[J]. SUSTAINABILITY,2022,14(19).
APA	Chen, Xiaojia.,Wu, Chengjian.,Li, Qinyu.,Zhou, Peng.,Chen, Zheng.,...&Zhao, Zhijun.(2022).Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks.SUSTAINABILITY,14(19).
MLA	Chen, Xiaojia,et al."Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks".SUSTAINABILITY 14.19(2022).