Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast

doi:10.1007/978-1-0716-0868-5_19

	Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast
其他题名	Yeast Protocols
	Zhang, Shanshan1,2 ; Li, Hui 3,4; Liu, Ji-Long1,4
著作类型	著
	2021
出版者	HUMANA PRESS INC
出版地	Switzerland
摘要	Live-cell imaging is widely used by researchers to study cellular dynamics and obtain a deep understanding of cell biological processes. Keeping cells in the proper growing environment and immobilizing the cells are essential for the imaging of live yeast cells. Here we describe a protocol for monitoring cytoophidia in Saccharomyces cerevisiae and Schizosaccharomyces pombe using inverted confocal fluorescence microscopy. This protocol includes yeast culture, sample preparation, fluorescence imaging, and data analysis.
关键词	Cytoophidium CTP synthase Live-cell imaging Long-term imaging Dynamic analysis Saccharomyces cerevisiae Schizosaccharomyces pombe
ISBN	978-1-0716-0867-8
DOI	10.1007/978-1-0716-0868-5_19
URL	查看原文
所属丛书	Methods in Molecular Biology
页数	235-244
语种	英语
文献类型	专著
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/243264
专题	生命科学与技术学院生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_博士生
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China; 3.Beijing Normal Univ, Sch Syst Sci, Beijing, Peoples R China; 4.Univ Oxford, Dept Physiol Anat & Genet, Oxford, England
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Shanshan,Li, Hui,Liu, Ji-Long. Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast[M]. Switzerland:HUMANA PRESS INC,2021.