CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function

doi:10.1007/978-1-0716-0266-9_5

	CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function
	Shang, Wanjing1,2,3 ; Wang, Fei1 ; Zhu, Qi1 ; Wang, Liangyu1 ; Wang, Haopeng1
	2020
发表期刊	T-CELL RECEPTOR SIGNALING: METHODS AND PROTOCOLS
ISSN	1064-3745
EISSN	1940-6029
卷号	2111 页码:59-70
发表状态	已发表
DOI	10.1007/978-1-0716-0266-9_5
摘要	T-cell-based cancer immunotherapies have emerged as a promising approach for cancer treatment, highlighting the importance of understanding the regulation of T-cell function. However, the molecular mechanisms underlying T-cell activation are not fully understood. The CRISPR/Cas9 system can serve as a robust method to systematically study signaling pathways. In this chapter, we describe details of using the CRISPR screen to identify regulators in TCR signaling, from the sgRNA library construction to genomic DNA sequencing. We also add some notes to further help readers performing the CRISPR screen. This approach can be readily adapted to study the activation of other immune cells, including B cells and dendritic cells.
关键词	gRNA library T-cell activation Lentivirus production and titer Lentiviral transduction Cell sorting Genomic DNA extraction
收录类别	BSCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology ; Immunology
WOS类目	Biochemical Research Methods ; Cell Biology ; Immunology
WOS记录号	WOS:000674446600006
出版者	HUMANA PRESS INC
原始文献类型	Article; Book Chapter
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/243188
专题	生命科学与技术学院生命科学与技术学院_PI研究组_王皞鹏组生命科学与技术学院_博士生生命科学与技术学院_本科生
通讯作者	Shang, Wanjing
作者单位	1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Shang, Wanjing,Wang, Fei,Zhu, Qi,et al. CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function[J]. T-CELL RECEPTOR SIGNALING: METHODS AND PROTOCOLS,2020,2111:59-70.
APA	Shang, Wanjing,Wang, Fei,Zhu, Qi,Wang, Liangyu,&Wang, Haopeng.(2020).CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function.T-CELL RECEPTOR SIGNALING: METHODS AND PROTOCOLS,2111,59-70.
MLA	Shang, Wanjing,et al."CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function".T-CELL RECEPTOR SIGNALING: METHODS AND PROTOCOLS 2111(2020):59-70.