Telomere Recombination Preferentially Occurs at Short Telomeres in Telomerase-Null Type II Survivors

doi:10.1371/journal.pone.0090644

	Telomere Recombination Preferentially Occurs at Short Telomeres in Telomerase-Null Type II Survivors
	Fu, Xiao-Hong 1; Duan, Yi-Min 1; Liu, Yu-Ting 1; Cai, Chen2 ; Meng, Fei-Long 1; Zhou, Jin-Qiu1
	2014-03-03
发表期刊	PLOS ONE (IF:2.9[JCR-2023],3.3[5-Year])
ISSN	1932-6203
卷号	9 期号:3
发表状态	已发表
DOI	10.1371/journal.pone.0090644
摘要	In telomerase negative yeast cells, Rad52-dependent recombination is activated to maintain telomeres. This recombination-mediated telomere elongation usually involves two independent pathways, type I and type II, and leads to generation of type I and type II survivors. It remains elusive whether the recombination-mediated telomere elongation prefers to take place on shorter or longer telomeres. In this study, we exploited the de novo telomere addition system to examine the telomere recombination event in telomerase negative cells. We show that recombination preferentially occurs on shorter rather than longer telomeres in both pre-survivors and established type II survivors. In type II survivors, the short VII-L telomeres could invade either terminal TG(1-3) sequence or short tracts of TG(1-3) sequence in subtelomeric Y'-X and Y'-Y' junction to initiate recombination. Unexpectedly, short VII-L telomere recombination still takes place in type II survivors lacking either Rad50 or Rad59, which are required for type II survivor generation in senescing telomerase-null cells. Our results support the notion that Rad50 and Rad59 are not essential for the maintenance of type II survivors once established.
收录类别	SCI
语种	英语
资助项目	Ministry of Science and Technology of China[2013CB910403] ; Ministry of Science and Technology of China[2011CB966301]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000332468900128
出版者	PUBLIC LIBRARY SCIENCE
WOS关键词	SACCHAROMYCES-CEREVISIAE ; YEAST TELOMERES ; REPLICATION ; DNA ; ELONGATION ; SENESCENCE ; MAINTENANCE ; PROTEIN ; TEL1P ; POLYMERASES
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2416
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_周金秋组生命科学与技术学院_博士生
通讯作者	Zhou, Jin-Qiu
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Univ Chinese Acad Sci, State Key Lab Mol Biol,Shanghai Inst Biochem & Ce, Shanghai, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Fu, Xiao-Hong,Duan, Yi-Min,Liu, Yu-Ting,et al. Telomere Recombination Preferentially Occurs at Short Telomeres in Telomerase-Null Type II Survivors[J]. PLOS ONE,2014,9(3).
APA	Fu, Xiao-Hong,Duan, Yi-Min,Liu, Yu-Ting,Cai, Chen,Meng, Fei-Long,&Zhou, Jin-Qiu.(2014).Telomere Recombination Preferentially Occurs at Short Telomeres in Telomerase-Null Type II Survivors.PLOS ONE,9(3).
MLA	Fu, Xiao-Hong,et al."Telomere Recombination Preferentially Occurs at Short Telomeres in Telomerase-Null Type II Survivors".PLOS ONE 9.3(2014).