Two intrinsic timing mechanisms set start and end times for dendritic arborization of a nociceptive neuron

doi:10.1073/pnas.2210053119

	Two intrinsic timing mechanisms set start and end times for dendritic arborization of a nociceptive neuron
	Suzuki, Nobuko 1; Zou, Yan1,2 ; Sun, HaoSheng 3; Eichel, Kelsie 4,5; Shao, Meiyu2 ; Shih, Mushaine 1; Shen, Kang 4,5; Chang, Chieh 1
	2022-11-02
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
EISSN	1091-6490
卷号	119 期号:45 页码:e2210053119
发表状态	已发表
DOI	10.1073/pnas.2210053119
摘要	Choreographic dendritic arborization takes place within a defined time frame, but the timing mechanism is currently not known. Here, we report that the precisely timed lin-4-lin-14 regulatory circuit triggers an initial dendritic growth activity, whereas the precisely timed lin-28-let-7-lin-41 regulatory circuit signals a subsequent developmental decline in dendritic growth ability, hence restricting dendritic arborization within a set time frame. Loss-of-function mutations in the lin-4 microRNA gene cause limited dendritic outgrowth, whereas loss-of-function mutations in its direct target, the lin-14 transcription factor gene, cause precocious and excessive outgrowth. In contrast, loss-of-function mutations in the let-7 microRNA gene prevent a developmental decline in dendritic growth ability, whereas loss-of-function mutations in its direct target, the lin-41 tripartite motif protein gene, cause further decline. lin-4 and let-7 regulatory circuits are expressed in the right place at the right time to set start and end times for dendritic arborization. Replacing the lin-4 upstream cis-regulatory sequence at the lin-4 locus with a late-onset let-7 upstream cis-regulatory sequence delays dendrite arborization, whereas replacing the let-7 upstream cis-regulatory sequence at the let-7 locus with an early-onset lin-4 upstream cis-regulatory sequence causes a precocious decline in dendritic growth ability. Our results indicate that the lin-4-lin-14 and the lin-28-let-7-lin-41 regulatory circuits control the timing of dendrite arborization through antagonistic regulation of the DMA-1 receptor level on dendrites. The LIN-14 transcription factor likely directly represses dma-1 gene expression through a transcriptional means, whereas the LIN-41 tripartite motif protein likely indirectly promotes dma-1 gene expression through a posttranscriptional means.
关键词	lin-4-lin-14 pathway lin-28-let-7-lin-41 pathway heterochronic genes neuronal timers dendrite arborization
URL	查看原文
收录类别	SCI
语种	英语
资助项目	NSF[IOS-1455758] ; National Institute of General Medical Sciences of the NIH[R01GM111320]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000907643500043
出版者	NATL ACAD SCIENCES
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/241052
专题	生命科学与技术学院_PI研究组_邹燕组生命科学与技术学院_硕士生
通讯作者	Chang, Chieh
作者单位	1.Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Univ Alabama Birmingham, Dept Cell Dev & Integrat Biol, Birmingham, AL 35233 USA 4.Stanford Univ, HHMI, Stanford, CA 94305 USA 5.Stanford Univ, Dept Biol, Stanford, CA 94305 USA
推荐引用方式 GB/T 7714	Suzuki, Nobuko,Zou, Yan,Sun, HaoSheng,et al. Two intrinsic timing mechanisms set start and end times for dendritic arborization of a nociceptive neuron[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(45):e2210053119.
APA	Suzuki, Nobuko.,Zou, Yan.,Sun, HaoSheng.,Eichel, Kelsie.,Shao, Meiyu.,...&Chang, Chieh.(2022).Two intrinsic timing mechanisms set start and end times for dendritic arborization of a nociceptive neuron.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(45),e2210053119.
MLA	Suzuki, Nobuko,et al."Two intrinsic timing mechanisms set start and end times for dendritic arborization of a nociceptive neuron".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.45(2022):e2210053119.