Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease

doi:10.1016/j.freeradbiomed.2014.02.024

	Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease
	Huang, Yijing 1; Zhu, Mingjiang 2,3; Li, Zi 4; Sa, Rina 2,5; Chu, Qianqian 2,3; Zhang, Qingli 4; Zhang, Haifeng 1; Tang, Wen 1; Zhang, Meifang 1; Yin, Huiyong2,3,6
	2014-05
发表期刊	FREE RADICAL BIOLOGY AND MEDICINE (IF:7.1[JCR-2023],7.9[5-Year])
ISSN	0891-5849
卷号	70 页码:223-232
发表状态	已发表
DOI	10.1016/j.freeradbiomed.2014.02.024
摘要	Periodontal diseases represent the most common chronic inflammatory diseases in humans and a major cause of tooth loss. Combining mass spectrometry-based ionomics and targeted lipidomics on fatty acid metabolites, we identified significant alterations in redox status and fatty acid metabolism in saliva in response to chronic inflammation and oxidative stress in periodontal disease in a cohort of nonsmoker subjects with chronic periodontitis. For the first time, ionomic profiling of around 30 ions in saliva revealed significantly decreased levels of redox-active metal ions including Mn, Cu, and Zn in the periodontal group, which is consistent with decreased levels of superoxide dismutases in saliva and serum. A targeted lipidomic approach was employed to monitor the major metabolites of arachidonic acid and linoleic acid in saliva. We observed increased levels of cyclooxygenase products including PGE(2), PGD(2), and PGF(2 alpha) and TXB2, but decreased level of PGI(2) in the periodontal group. A unique pattern of the lipoxygenase products of arachidonic acid and linoleic acid was observed with increased level of 5-HETE but decreased levels of 13-HODE and 9-HODE. Levels of salivary F-2-isoprostanes, free radical lipid peroxidation products, and a gold standard for oxidative stress in vivo were also significantly elevated. Taking these data together, our study using multiple powerful omics techniques demonstrates that local redox alteration contributes significantly to periodontitis through the modulation of fatty acid metabolism in response to inflammation and oxidative stress. This study highlights the importance of redox status in periodontitis and provides a rationale for preventing periodontal disease by dietary interventions aiming to restore redox balance. (C) 2014 Elsevier Inc. All rights reserved.
关键词	Periodontitis Saliva Fatty acid metabolism Ionomic Lipidomics Redox status Oxidative stress Inflammation Free radicals
收录类别	SCI
语种	英语
资助项目	Hundred Talents Program from the Chinese Academy of Sciences[2012OHTP07]
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS记录号	WOS:000335487100022
出版者	ELSEVIER SCIENCE INC
WOS关键词	GINGIVAL CREVICULAR FLUID ; OLDER CHINESE ; EICOSANOID BIOLOGY ; ARACHIDONIC-ACID ; RISK ; F-2-ISOPROSTANES ; MECHANISMS ; PHOSPHOLIPIDS ; DEGRADATION ; APOPTOSIS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2407
专题	生命科学与技术学院_特聘教授组_尹慧勇组
通讯作者	Yin, Huiyong
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Clin Nutr, Shanghai 200030, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Food Safety Res, Shanghai 200031, Peoples R China 3.Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Mass Spectrometry Res Ctr, Shanghai 200031, Peoples R China 5.Univ Chinese Acad Sci, Shanghai, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Huang, Yijing,Zhu, Mingjiang,Li, Zi,et al. Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease[J]. FREE RADICAL BIOLOGY AND MEDICINE,2014,70:223-232.
APA	Huang, Yijing.,Zhu, Mingjiang.,Li, Zi.,Sa, Rina.,Chu, Qianqian.,...&Yin, Huiyong.(2014).Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease.FREE RADICAL BIOLOGY AND MEDICINE,70,223-232.
MLA	Huang, Yijing,et al."Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease".FREE RADICAL BIOLOGY AND MEDICINE 70(2014):223-232.