Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia

doi:10.3390/ijms231911698

	Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
	Fang, Yi-Fan1 ; Li, Yi-Lan1 ; Li, Xiao-Ming1 ; Liu, Ji-Long1,2
	2022-10
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (IF:4.9[JCR-2023],5.6[5-Year])
EISSN	1422-0067
卷号	23 期号:19
发表状态	已发表
DOI	10.3390/ijms231911698
摘要	CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components, such as IMPDH. In addition, we observe CTPS granules with tentacles.
关键词	CTP synthase cytoophidium fluorescence recovery after photobleaching (FRAP) stimulated emission depletion (STED)
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2021YFA0804700] ; National Natural Science Foundation of China[31771490] ; Shanghai Science and Technology Commission[20JC1410500] ; UK Medical Research Council["MC_UU_12021/3","MC_U137788471"]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000867848100001
出版者	MDPI
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/240478
专题	生命科学与技术学院_本科生生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_公共科研平台_分子影像平台生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Liu, Ji-Long
作者单位	1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT, England
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Fang, Yi-Fan,Li, Yi-Lan,Li, Xiao-Ming,et al. Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(19).
APA	Fang, Yi-Fan,Li, Yi-Lan,Li, Xiao-Ming,&Liu, Ji-Long.(2022).Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(19).
MLA	Fang, Yi-Fan,et al."Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.19(2022).