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Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry
2014-09-12
发表期刊JOURNAL OF CHROMATOGRAPHY A (IF:3.8[JCR-2023],3.5[5-Year])
ISSN0021-9673
卷号1359页码:84-90
发表状态已发表
DOI10.1016/j.chroma.2014.07.020
摘要A new approach for proteome-wide profiling drug binding proteins by using monolithic capillary affinity chromatography in combination with HPLC-MS/MS is reported. Two immunosuppresive drugs, namely FK506 and cyclosporin A, were utilized as the experimental models for proof-of-concept. The monolithic capillary affinity columns were prepared through a single-step copolymerization of the drug derivatives with glycidyl methacrylate and ethylene dimethacrylate. The capillary chromatography with the affinity monolithic column facilitates the purification of the drug binding proteins from the cell lysate. By combining the capillary affinity column purification and the shot-gun proteomic analysis, totally 33 FK506- and 32 CsA-binding proteins including all the literature reported target proteins of these two drugs were identified. Among them, two proteins, namely voltage-dependent anion-selective channel protein 1 and serine/threonine-protein phosphatase PGAM5 were verified by using the recombinant proteins. The result supports that the monolithic capillary affinity chromatography is likely to become a valuable tool for profiling of binding proteins of small molecular drugs as well as bioactive compounds. (C) 2014 Elsevier B.V. All rights reserved.
关键词Monolithic column Affinity chromatography Drug target identification Chemical proteomics
收录类别SCI ; EI
语种英语
资助项目National Natural Science Foundations of China[21375140] ; National Natural Science Foundations of China[21175146] ; National Natural Science Foundations of China[90713021] ; National Natural Science Foundations of China[81071668]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000340984400011
出版者ELSEVIER SCIENCE BV
EI入藏号20143418090488
EI主题词Acrylic monomers ; Affinity chromatography ; Amino acids ; Column chromatography ; Ethylene ; Liquid chromatography ; Mass spectrometry ; Purification
EI分类号Chemistry:801 ; Chemical Operations:802.3 ; Organic Compounds:804.1
WOS关键词TARGET IDENTIFICATION ; CHEMICAL GENETICS ; SILICA COLUMNS ; CALCINEURIN ; PHOSPHATASE ; SEPARATION ; DASATINIB ; KINASE ; FK506
原始文献类型Article
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2372
专题物质科学与技术学院_特聘教授组_康经武组
通讯作者Kang, Jingwu
作者单位
1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Organ & Nat Prod Chem, Shanghai 200032, Peoples R China
2.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Key Lab Cell Differentiat & Apoptosis,Chinese Min, Shanghai 200025, Peoples R China
3.ShanghaiTech Univ, Shanghai 200031, Peoples R China
通讯作者单位上海科技大学
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GB/T 7714
Zhang, Xuepei,Wang, Tongdan,Zhang, Hanzhi,et al. Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1359:84-90.
APA Zhang, Xuepei,Wang, Tongdan,Zhang, Hanzhi,Han, Bing,Wang, Lishun,&Kang, Jingwu.(2014).Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1359,84-90.
MLA Zhang, Xuepei,et al."Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1359(2014):84-90.
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