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ShanghaiTech University Knowledge Management System
Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry | |
2014-09-12 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHY A (IF:3.8[JCR-2023],3.5[5-Year]) |
ISSN | 0021-9673 |
卷号 | 1359页码:84-90 |
发表状态 | 已发表 |
DOI | 10.1016/j.chroma.2014.07.020 |
摘要 | A new approach for proteome-wide profiling drug binding proteins by using monolithic capillary affinity chromatography in combination with HPLC-MS/MS is reported. Two immunosuppresive drugs, namely FK506 and cyclosporin A, were utilized as the experimental models for proof-of-concept. The monolithic capillary affinity columns were prepared through a single-step copolymerization of the drug derivatives with glycidyl methacrylate and ethylene dimethacrylate. The capillary chromatography with the affinity monolithic column facilitates the purification of the drug binding proteins from the cell lysate. By combining the capillary affinity column purification and the shot-gun proteomic analysis, totally 33 FK506- and 32 CsA-binding proteins including all the literature reported target proteins of these two drugs were identified. Among them, two proteins, namely voltage-dependent anion-selective channel protein 1 and serine/threonine-protein phosphatase PGAM5 were verified by using the recombinant proteins. The result supports that the monolithic capillary affinity chromatography is likely to become a valuable tool for profiling of binding proteins of small molecular drugs as well as bioactive compounds. (C) 2014 Elsevier B.V. All rights reserved. |
关键词 | Monolithic column Affinity chromatography Drug target identification Chemical proteomics |
收录类别 | SCI ; EI |
语种 | 英语 |
资助项目 | National Natural Science Foundations of China[21375140] ; National Natural Science Foundations of China[21175146] ; National Natural Science Foundations of China[90713021] ; National Natural Science Foundations of China[81071668] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000340984400011 |
出版者 | ELSEVIER SCIENCE BV |
EI入藏号 | 20143418090488 |
EI主题词 | Acrylic monomers ; Affinity chromatography ; Amino acids ; Column chromatography ; Ethylene ; Liquid chromatography ; Mass spectrometry ; Purification |
EI分类号 | Chemistry:801 ; Chemical Operations:802.3 ; Organic Compounds:804.1 |
WOS关键词 | TARGET IDENTIFICATION ; CHEMICAL GENETICS ; SILICA COLUMNS ; CALCINEURIN ; PHOSPHATASE ; SEPARATION ; DASATINIB ; KINASE ; FK506 |
原始文献类型 | Article |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2372 |
专题 | 物质科学与技术学院_特聘教授组_康经武组 |
通讯作者 | Kang, Jingwu |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Organ & Nat Prod Chem, Shanghai 200032, Peoples R China 2.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Key Lab Cell Differentiat & Apoptosis,Chinese Min, Shanghai 200025, Peoples R China 3.ShanghaiTech Univ, Shanghai 200031, Peoples R China |
通讯作者单位 | 上海科技大学 |
推荐引用方式 GB/T 7714 | Zhang, Xuepei,Wang, Tongdan,Zhang, Hanzhi,et al. Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1359:84-90. |
APA | Zhang, Xuepei,Wang, Tongdan,Zhang, Hanzhi,Han, Bing,Wang, Lishun,&Kang, Jingwu.(2014).Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1359,84-90. |
MLA | Zhang, Xuepei,et al."Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1359(2014):84-90. |
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