Next-Generation Models of Human Cardiogenesis via Genome Editing

doi:10.1101/cshperspect.a013920

	Next-Generation Models of Human Cardiogenesis via Genome Editing
	Lian, Xiaojun 1; Xu, Jiejia 1; Li, Jinsong2,3 ; Chien, Kenneth R.1,4
	2014-12
发表期刊	COLD SPRING HARBOR PERSPECTIVES IN MEDICINE (IF:7.8[JCR-2023],9.1[5-Year])
ISSN	2157-1422
卷号	4 期号:12
发表状态	已发表
DOI	10.1101/cshperspect.a013920
摘要	Cardiogenesis is one of the earliest and most important steps during human development and is orchestrated by discrete families of heart progenitors, which build distinct regions of the fetal heart. For the past decade, a lineage map for the distinct subsets of progenitors that generate the embryonic mammalian heart has begun to lay a foundation for the development of new strategies for rebuilding the adult heart after injury, an unmet clinical need for the vast majority of patients with end-stage heart failure who are not heart transplant recipients. The studies also have implications for the root causes of congenital heart disease, which affects 1 in 50 live births, the most prevalent malformations in children. Although much of this insight has been generated in murine models, it is becoming increasingly clear that there can be important divergence with principles and pathways for human cardiogenesis, as well as for regenerative pathways. The development of human stem cell models, coupled with recent advances in genome editing with RNA-guided endonucleases, offers a new approach for the primary study of human cardiogenesis. In addition, application of the technology to the in vivo setting in large animal models, including nonhuman primates, has opened the door to genome-edited large animal models of adult and congenital heart disease, as well as a detailed mechanistic dissection of the more diverse and complex set of progenitor families and pathways, which guide human cardiogenesis. Implications of this new technology for a new generation of human-based, genetically tractable systems are discussed, along with potential therapeutic applications.
收录类别	SCI
语种	英语
WOS研究方向	Research & Experimental Medicine
WOS类目	Medicine, Research & Experimental
WOS记录号	WOS:000346523200001
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS关键词	EMBRYONIC STEM-CELLS ; ENDOTHELIAL-CELLS ; CARDIAC DIFFERENTIATION ; CARDIOMYOCYTE DIFFERENTIATION ; CONDITIONAL ALLELES ; HEART PROGENITORS ; GENETIC SCREENS ; RNAI SCREEN ; MOUSE ; MODULATION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2356
专题	生命科学与技术学院_特聘教授组_李劲松组
通讯作者	Chien, Kenneth R.
作者单位	1.Karolinska Inst, Dept Cell & Mol Biol, S-17177 Solna, Sweden 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Grp Epigenet Reprogrammi, Shanghai Key Lab Mol Androl,State Key Lab Cell Bi, Shanghai 200031, Peoples R China 3.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 4.Karolinska Inst, Dept Med, S-14186 Huddinge, Sweden
推荐引用方式 GB/T 7714	Lian, Xiaojun,Xu, Jiejia,Li, Jinsong,et al. Next-Generation Models of Human Cardiogenesis via Genome Editing[J]. COLD SPRING HARBOR PERSPECTIVES IN MEDICINE,2014,4(12).
APA	Lian, Xiaojun,Xu, Jiejia,Li, Jinsong,&Chien, Kenneth R..(2014).Next-Generation Models of Human Cardiogenesis via Genome Editing.COLD SPRING HARBOR PERSPECTIVES IN MEDICINE,4(12).
MLA	Lian, Xiaojun,et al."Next-Generation Models of Human Cardiogenesis via Genome Editing".COLD SPRING HARBOR PERSPECTIVES IN MEDICINE 4.12(2014).