Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe

doi:10.1016/j.yexcr.2022.113337

	Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe
	Andreadis, Christos1,3 ; Li, Tianhao1 ; Liu, Ji-Long1,2
	2022-11-01
发表期刊	EXPERIMENTAL CELL RESEARCH (IF:3.3[JCR-2023],3.3[5-Year])
ISSN	0014-4827
EISSN	1090-2422
卷号	420 期号:1
发表状态	已发表
DOI	10.1016/j.yexcr.2022.113337
摘要	CTP synthase (CTPS), a metabolic enzyme responsible for the de novo synthesis of CTP, can form filamentous structures termed cytoophidia, which are evolutionarily conserved from bacteria to humans. Here we used Schizosaccharomyces pombe to study the cytoophidium assembly regulation by ubiquitination. We tested the CTP synthase's capacity to be post-translationally modified by ubiquitin or be affected by the ubiquitination state of the cell and showed that ubiquitination is important for the maintenance of the CTPS filamentous structure in fission yeast. We have identified proteins which are in complex with CTPS, including specific ubiquitination regulators which significantly affect CTPS filamentation, and mapped probable ubiquitination targets on CTPS. Furthermore, we discovered that a cohort of deubiquitinating enzymes is important for the regulation of cytoophidium's filamentous morphology. Our study provides a framework for the analysis of the effects that ubiquitination and deubiquitination have on the formation of cytoophidia.
关键词	CTP synthase Cytoophidium Ubiquitination Filament Schizosaccharomyces pombe
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2021YFA0804700] ; National Natural Science Foundation of China[31771490] ; Shanghai Science and Technology Commission[20JC1410500] ; UK Medical Research Council["MC_UU_12,021/3","MC_U137788471"]
WOS研究方向	Oncology ; Cell Biology
WOS类目	Oncology ; Cell Biology
WOS记录号	WOS:000864095900001
出版者	ELSEVIER INC
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/231948
专题	生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_博士生
通讯作者	Liu, Ji-Long
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 2.Univ Oxford, Dept Physiol Anat & Genet, Oxford OX1 3PT, England 3.Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Andreadis, Christos,Li, Tianhao,Liu, Ji-Long. Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe[J]. EXPERIMENTAL CELL RESEARCH,2022,420(1).
APA	Andreadis, Christos,Li, Tianhao,&Liu, Ji-Long.(2022).Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe.EXPERIMENTAL CELL RESEARCH,420(1).
MLA	Andreadis, Christos,et al."Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe".EXPERIMENTAL CELL RESEARCH 420.1(2022).