Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep

doi:10.1186/s12864-018-4712-z

	Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep
	Wang, Xiaolong 1; Liu, Jing 1; Niu, Yiyuan 1; Li, Yan 1; Zhou, Shiwei 1; Li, Chao 1; Ma, Baohua 2; Kou, Qifang 3; Petersen, Bjoern 4; Sonstegard, Tad 5; Huang, Xingxu6 ; Jiang, Yu 1; Chen, Yulin 1
	2018-05-25
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	19
发表状态	已发表
DOI	10.1186/s12864-018-4712-z
摘要	Background: The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended mutations produced by off-target CRISPR/Cas9 nuclease activity may lead to negative consequences. Especially, a very recent study found that gene editing can introduce hundreds of unintended mutations into the genome, and have attracted wide attention.
关键词	Genome editing CRISPR/Cas9 Whole genome sequencing Off-target mutation rate Sheep
收录类别	SCI ; SCIE
语种	英语
资助项目	China Agriculture Research System[CARS-39]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000433331200001
出版者	BMC
WOS关键词	OFF-TARGET MUTATIONS ; DE-NOVO MUTATIONS ; CRISPR-CAS9 ; CAS9 ; INVERSIONS ; GENERATION ; ALGORITHM ; FRAMEWORK ; MODEL
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/23071
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Huang, Xingxu; Jiang, Yu; Chen, Yulin
作者单位	1.Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China 2.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China 3.Ningxia Tianyuan Tan Sheep Farm, Hongsibu 751999, Peoples R China 4.Friedrich Loeffler Inst, Inst Farm Anim Genet, D-31535 Neustadt, Germany 5.Recombinetics, Minneapolis, MN 55104 USA 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Xiaolong,Liu, Jing,Niu, Yiyuan,et al. Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep[J]. BMC GENOMICS,2018,19.
APA	Wang, Xiaolong.,Liu, Jing.,Niu, Yiyuan.,Li, Yan.,Zhou, Shiwei.,...&Chen, Yulin.(2018).Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep.BMC GENOMICS,19.
MLA	Wang, Xiaolong,et al."Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep".BMC GENOMICS 19(2018).