Biological Functions and Cross-Kingdom Host Gene Regulation of Small RNAs in Lactobacillus plantarum-Derived Extracellular Vesicles

doi:10.3389/fmicb.2022.944361

	Biological Functions and Cross-Kingdom Host Gene Regulation of Small RNAs in Lactobacillus plantarum-Derived Extracellular Vesicles
	Yu, Siran 1,2; Zhao, Zhehao 1,2; Hao, Piliang3 ; Qiu, Yan 1,2; Zhao, Meiyi 1,2; Zhou, Gang 1,2; Zhang, Chengqian3 ; Kang, Jiuhong 1,2; Li, Ping 1,2
	2022-08-18
发表期刊	FRONTIERS IN MICROBIOLOGY (IF:4.0[JCR-2023],5.1[5-Year])
EISSN	1664-302X
卷号	13
发表状态	已发表
DOI	10.3389/fmicb.2022.944361
摘要	Extracellular vesicle-mediated transfer of microRNAs is a novel mode of cell-to-cell genetic transmission. Extracellular vesicles produced by microbes have been shown to contain significant quantities of physiologically active molecules such as proteins, lipids, and RNA, which could be transported to host cells and play a key role in both inter-kingdom signaling and physiological responses. In this study, we identified sRNAs by sequencing small RNAs (sRNAs) from Lactobacillus plantarum-derived extracellular vesicles (LDEVs) and detected the expression levels of vesicular sRNAs using quantitative reverse transcription-polymerase chain reaction (RT-PCR), which demonstrated the presence of microRNA-sized RNAs (msRNAs) within these vesicles. We chose sRNA71, a highly expressed msRNA, for further investigation, predicted its potential target genes for the human genome, and indicated that it could be translocated into mammalian cells. The biological functions of this sRNA71 were subsequently explored through cellular proteomics, western blot, and luciferase reporter assay. According to the findings, transfection with synthetic sRNA71 mimics substantially reduced Tp53 expression in HEK293T cells and suppressed the gene expression through binding to the 3 ' UTR of Tp53 mRNA. In conclusion, it is hypothesized that microbial-derived extracellular vesicles serve as carriers of functional molecules such as sRNAs, which play an essential role in regulating microbial-host communication.
关键词	microRNA microRNA-size RNA (msRNA) lactobacillus-derived extracellular vesicles (LDEVs) post-transcriptional regulation microbe-host interspecies communication
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收录类别	SCI ; SCIE
语种	英语
资助项目	[21878236] ; [U19A2034]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000848480100001
出版者	FRONTIERS MEDIA SA
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/226375
专题	生命科学与技术学院_公共科研平台_组学分析平台
通讯作者	Kang, Jiuhong; Li, Ping
作者单位	1.Tongji Univ, Clin & Translat Res Ctr Shanghai Matern 1, Shanghai, Peoples R China 2.Tongji Univ, Infant Hosp, Sch Life Sci & Technol, Shanghai, Chin, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Yu, Siran,Zhao, Zhehao,Hao, Piliang,et al. Biological Functions and Cross-Kingdom Host Gene Regulation of Small RNAs in Lactobacillus plantarum-Derived Extracellular Vesicles[J]. FRONTIERS IN MICROBIOLOGY,2022,13.
APA	Yu, Siran.,Zhao, Zhehao.,Hao, Piliang.,Qiu, Yan.,Zhao, Meiyi.,...&Li, Ping.(2022).Biological Functions and Cross-Kingdom Host Gene Regulation of Small RNAs in Lactobacillus plantarum-Derived Extracellular Vesicles.FRONTIERS IN MICROBIOLOGY,13.
MLA	Yu, Siran,et al."Biological Functions and Cross-Kingdom Host Gene Regulation of Small RNAs in Lactobacillus plantarum-Derived Extracellular Vesicles".FRONTIERS IN MICROBIOLOGY 13(2022).