Survival Motor Neuron Enhances Pluripotent Gene Expression and Facilitates Cell Reprogramming

doi:10.1089/scd.2022.0091

	Survival Motor Neuron Enhances Pluripotent Gene Expression and Facilitates Cell Reprogramming
	Chang, Wei-Fang 1; Lin, Tzu-Ying 1; Peng, Min 1; Chang, Chia-Chun 1; Xu, Jie 2; Hsieh-Li, Hsiu-Mei 3; Liu, Ji-Long4,5 ; Sung, Li-Ying 1,6,7,8
	2022-08
发表期刊	STEM CELLS AND DEVELOPMENT (IF:2.5[JCR-2023],4.4[5-Year])
ISSN	1547-3287
EISSN	1557-8534
发表状态	已发表
DOI	10.1089/scd.2022.0091
摘要	Survival motor neuron (SMN) plays important roles in snRNP assembly and mRNA splicing. Deficiency of SMN causes spinal muscular atrophy (SMA), a leading genetic disease causing childhood mortality. Previous studies have shown that SMN regulates stem cell self-renewal and pluripotency in Drosophila and mouse and is abundantly expressed in mouse embryonic stem cells. However, whether SMN is required for establishment of pluripotency is unclear. In this study, we show that SMN is gradually upregulated in preimplantation mouse embryos and cultured cells undergoing cell reprogramming. Ectopic expression of SMN increased cell reprogramming efficiency, whereas knockdown of SMN impeded induced pluripotent stem cell (iPSC) colony formation. iPSCs could be derived from SMA model mice, but impairment in differentiation capacity may be present. The ectopic overexpression of SMN in iPSCs can upregulate the expression levels of some pluripotent genes and restore the neuronal differentiation capacity of SMA-iPSCs. Taken together, our findings not only demonstrate the functional relevance of SMN in establishment of cell pluripotency but also propose its potential application in facilitating iPSC derivation.
关键词	survival motor neuron spinal muscular atrophy induced pluripotent stem cells somatic cell reprogramming neuronal differentiation
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	[MOST 106-2313-B-002-039-MY3] ; [MOST 109-2313-B-002-003-MY2] ; [MOST110-2811-B-002-624]
WOS研究方向	Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
WOS类目	Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
WOS记录号	WOS:000844306200001
出版者	MARY ANN LIEBERT, INC
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/223047
专题	生命科学与技术学院_PI研究组_刘冀珑组
通讯作者	Sung, Li-Ying
作者单位	1.Natl Taiwan Univ, Inst Biotechnol, Taipei, Taiwan 2.Univ Michigan, Ctr Adv Models Translat Sci & Therapeut, Med Ctr, Ann Arbor, MI USA 3.Natl Taiwan Normal Univ, Dept Life Sci, Taipei, Taiwan 4.Univ Oxford, Dept Physiol Anat & Genet, MRC Funct Genom Unit, Oxford, England 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Acad Sinica, Agr Biotechnol Res Ctr, Taipei, Taiwan 7.Natl Taiwan Univ, Anim Resource Ctr, Taipei, Taiwan 8.Natl Taiwan Univ, Inst Biotechnol, 81 Chang Xiang St, Taipei 106, Taiwan
推荐引用方式 GB/T 7714	Chang, Wei-Fang,Lin, Tzu-Ying,Peng, Min,et al. Survival Motor Neuron Enhances Pluripotent Gene Expression and Facilitates Cell Reprogramming[J]. STEM CELLS AND DEVELOPMENT,2022.
APA	Chang, Wei-Fang.,Lin, Tzu-Ying.,Peng, Min.,Chang, Chia-Chun.,Xu, Jie.,...&Sung, Li-Ying.(2022).Survival Motor Neuron Enhances Pluripotent Gene Expression and Facilitates Cell Reprogramming.STEM CELLS AND DEVELOPMENT.
MLA	Chang, Wei-Fang,et al."Survival Motor Neuron Enhances Pluripotent Gene Expression and Facilitates Cell Reprogramming".STEM CELLS AND DEVELOPMENT (2022).