Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection

doi:10.1007/s10337-015-2906-y

	Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection
	Zhang, Mingyu 1; Qiu, Danye 1; Kang, Jingwu1,2
	2015-06
发表期刊	CHROMATOGRAPHIA (IF:1.2[JCR-2023],1.7[5-Year])
ISSN	0009-5893
卷号	78 期号:11-12 页码:833-837
发表状态	已发表
DOI	10.1007/s10337-015-2906-y
摘要	A simple, sensitive and environmentally friendly capillary electrophoresis method for the determination of free sulfate anions in heparin and low-molecular-weight heparin with indirect UV detection is reported. The background electrolyte consists of 20 mM nitrate (pH 5.5) containing 0.2 mM CTAB. The use of nitrate as the probe anion is based on the consideration that nitrate has a very close electrophoretic mobility to that of sulfate, leading to a very narrow peak shape. Moreover, the apparent molar absorptivity of nitrate at 202 nm (8413 L mol(-1) cm(-1)) is much higher than that of the commonly used probe anion chromate at 254 nm (2400 L mol(-1) cm(-1)). The combination of the narrow peak shape with the high apparent molar absorptivity of the probe ion improved the limit of detection by 2.4 times and the limit of quantitation by 3.2 times. The effect of various CE parameters on the separation of sulfate from chloride was investigated and optimized. The method displays linearity in the range from 0.05 to 20 mM. The RSD % for intraday (n = 6), and interday (n = 3) repeatability in terms of migration times and peak areas were all less than 3 %. The limit of detection (S/N = 3) and limit of quantitation (S/N = 10) for sulfate were determined as 18 and 53 A mu M, respectively. The method was successfully applied for the quantification of sulfate anions in heparin and low-molecular-weight heparins.
关键词	Capillary electrophoresis Indirect UV detection Sulfate anions Heparin
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundations of China[21375140] ; National Natural Science Foundations of China[21175146]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000354904100010
出版者	SPRINGER HEIDELBERG
WOS关键词	STABILITY ; WATER
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2205
专题	物质科学与技术学院_特聘教授组_康经武组
通讯作者	Kang, Jingwu
作者单位	1.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 2.ShanghaiTech Univ, Shanghai 200031, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Zhang, Mingyu,Qiu, Danye,Kang, Jingwu. Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection[J]. CHROMATOGRAPHIA,2015,78(11-12):833-837.
APA	Zhang, Mingyu,Qiu, Danye,&Kang, Jingwu.(2015).Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection.CHROMATOGRAPHIA,78(11-12),833-837.
MLA	Zhang, Mingyu,et al."Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection".CHROMATOGRAPHIA 78.11-12(2015):833-837.