Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations

doi:10.1186/s12858-015-0046-5

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	Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations
	Liu, Shu-su1 ; Wei, Xuan1 ; Dong, Xue1 ; Xu, Liang1 ; Liu, Jia1,2 ; Jiang, Biao1
	2015-07-25
发表期刊	BMC BIOCHEMISTRY
ISSN	1471-2091
卷号	16
发表状态	已发表
DOI	10.1186/s12858-015-0046-5
摘要	Background: Green fluorescent protein (GFP) and its derivative fluorescent proteins (FPs) are among the most commonly used reporter systems for studying gene expression and protein interaction in biomedical research. Most commercially available FPs have been optimized for their oligomerization state to prevent potential structural constraints that may interfere with the native function of fused proteins. Other approach to reducing structural constraints may include minimizing the structure of GFPs. Previous studies in an enhanced GFP variant (EGFP) identified a series of deletions that can retain GFP fluorescence. In this study, we interrogated the structural plasticity of a UV-optimized GFP variant (GFPUV) to amino acid deletions, characterized the effects of deletions and explored the feasibility of rescuing the fluorescence of deletion mutants using folding-enhancing mutations.
关键词	Green fluorescent protein (GFP) Transposon mutagenesis Amino acid deletions Protein folding Chromophore maturation
收录类别	SCI
语种	英语
资助项目	ShanghaiTech University, NIH[R01GM084396]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000358349000001
出版者	BIOMED CENTRAL LTD
WOS关键词	TEM-1 BETA-LACTAMASE ; DIRECTED EVOLUTION ; CRYSTAL-STRUCTURE ; RANDOM INSERTION ; AEQUOREA ; GFP ; MUTAGENESIS ; TRANSPOSONS ; CHROMOPHORE ; TOLERANCE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2172
专题	免疫化学研究所生命科学与技术学院免疫化学研究所_特聘教授组_抗体工程学实验室免疫化学研究所_特聘教授组_抗体化学实验室免疫化学研究所_公共科研平台生命科学与技术学院_博士生免疫化学研究所_公共科研平台_高通量筛选平台免疫化学研究所_PI研究组_刘佳组
通讯作者	Liu, Jia
作者单位	1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China 2.Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
第一作者单位	免疫化学研究所
通讯作者单位	免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Liu, Shu-su,Wei, Xuan,Dong, Xue,et al. Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations[J]. BMC BIOCHEMISTRY,2015,16.
APA	Liu, Shu-su,Wei, Xuan,Dong, Xue,Xu, Liang,Liu, Jia,&Jiang, Biao.(2015).Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations.BMC BIOCHEMISTRY,16.
MLA	Liu, Shu-su,et al."Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations".BMC BIOCHEMISTRY 16(2015).