R-acetoin accumulation and dissimilation in Klebsiella pneumoniae

doi:10.1007/s10295-015-1638-1

	R-acetoin accumulation and dissimilation in Klebsiella pneumoniae
	Wang, Dexin 1; Zhou, Jidong 1; Chen, Chuan 1; Wei, Dong 1; Shi, Jiping1,2 ; Jiang, Biao 1; Liu, Pengfu 1; Hao, Jian 1
	2015-08
发表期刊	JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (IF:3.2[JCR-2023],3.3[5-Year])
ISSN	1367-5435
卷号	42 期号:8 页码:1105-1115
发表状态	已发表
DOI	10.1007/s10295-015-1638-1
摘要	Klebsiella pneumoniae is a 2,3-butanediol producer, and R-acetoin is an intermediate of 2,3-butanediol production. R-acetoin accumulation and dissimilation in K. pneumoniae was studied here. A budC mutant, which has lost 2,3-butanediol dehydrogenase activity, accumulated high levels of R-acetoin in culture broth. However, after glucose was exhausted, the accumulated R-acetoin could be reused by the cells as a carbon source. Acetoin dehydrogenase enzyme system, encoded by acoABCD, was responsible for R-acetoin dissimilation. acoABCD mutants lost the ability to grow on acetoin as the sole carbon source, and the acetoin accumulated could not be dissimilated. However, in the presence of another carbon source, the acetoin accumulated in broth of acoABCD mutants was converted to 2,3-butanediol. Parameters of R-acetoin production by budC mutants were optimized in batch culture. Aerobic culture and mildly acidic conditions (pH 6-6.5) favored R-acetoin accumulation. At the optimized conditions, in fed-batch fermentation, 62.3 g/L R-acetoin was produced by budC and acoABCD double mutant in 57 h culture, with an optical purity of 98.0 %, and a substrate conversion ratio of 28.7 %.
关键词	R-acetoin Acetoin dehydrogenase enzyme system 2,3-Butanediol dehydrogenase Klebsiella pneumoniae
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[20906076]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000358291900002
出版者	SPRINGER HEIDELBERG
WOS关键词	SERRATIA-MARCESCENS H32 ; STATISTICAL OPTIMIZATION ; EFFICIENT PRODUCTION ; MEDIUM COMPONENTS ; GENE REPLACEMENT ; IDENTIFICATION ; FERMENTATION ; PATHWAY ; DEHYDROGENASE ; METABOLISM
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2169
专题	生命科学与技术学院_特聘教授组_史吉平组
通讯作者	Hao, Jian
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Dexin,Zhou, Jidong,Chen, Chuan,et al. R-acetoin accumulation and dissimilation in Klebsiella pneumoniae[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(8):1105-1115.
APA	Wang, Dexin.,Zhou, Jidong.,Chen, Chuan.,Wei, Dong.,Shi, Jiping.,...&Hao, Jian.(2015).R-acetoin accumulation and dissimilation in Klebsiella pneumoniae.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(8),1105-1115.
MLA	Wang, Dexin,et al."R-acetoin accumulation and dissimilation in Klebsiella pneumoniae".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.8(2015):1105-1115.