CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse
Zi, Zhenzhen1,2; Zhang, Zhuzhen3; Li, Qingrun3; An, Weiwei3; Zeng, Liyong4; Gao, Dayuan5; Yang, Ying3; Zhu, Xueliang4; Zeng, Rong3,5; Shum, Winnie Waichi5; Wu, Jiarui1,2,3,5,6
2015-08
Source PublicationPLOS GENETICS
ISSN1553-7404
Volume11Issue:8
Status已发表
DOI10.1371/journal.pgen.1005485
AbstractCyclin Y-like 1 (Ccnyl1) is a newly-identified member of the cyclin family and is highly similar in protein sequences to Cyclin Y (Ccny). However, the function of Ccnyl1 is poorly characterized in any organism. Here we found that Ccnyl1 was most abundantly expressed in the testis of mice and was about seven times higher than the level of Ccny. Male Ccnyl1-/- mice were infertile, whereas both male and female Ccny-/- mice displayed normal fertility. These results suggest that Ccnyl1, but not Ccny, is indispensable for male fertility. Spermatozoa obtained from Ccnyl1-/- mice displayed significantly impaired motility, and represented a thinned annulus region and/or a bent head. We found that the protein, but not the mRNA, level of cyclin-dependent kinase 16 (CDK16) was decreased in the testis of Ccnyl1-/- mice. Further study demonstrated that CCNYL1 interacted with CDK16 and this interaction mutually increased the stability of these two proteins. Moreover, the interaction increased the kinase activity of CDK16. In addition, we observed an alteration of phosphorylation levels of CDK16 in the presence of CCNYL1. We identified the phosphorylation sites of CDK16 by mass spectrometry and revealed that several phosphorylation modifications on the N-terminal region of CDK16 were indispensable for the CCNYL1 binding and the modulation of CDK16 kinase activity. Our results therefore reveal a previously unrecognized role of CCNYL1 in regulating spermatogenesis through the interaction and modulation of CDK16.
Indexed BySCI
Language英语
Funding ProjectScience and Technology Commission of Shanghai Municipality[13DZ1940100]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000360823100059
PublisherPUBLIC LIBRARY SCIENCE
WOS KeywordSPERM TERMINAL DIFFERENTIATION ; CELL-CYCLE ; PROTEIN-KINASES ; MAMMALIAN SPERMATOGENESIS ; PHOSPHORYLATION ; ACTIVATION ; PCTAIRE-1 ; FAMILY ; ORGANIZATION ; EXOCYTOSIS
Original Document TypeArticle
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2161
Collection生命科学与技术学院_博士生
生命科学与技术学院
生命科学与技术学院_PI研究组_岑慧枝组
生命科学与技术学院_特聘教授组_曾嵘组
Corresponding AuthorZi, Zhenzhen
Affiliation1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
3.Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China
4.Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
6.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Zi, Zhenzhen,Zhang, Zhuzhen,Li, Qingrun,et al. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse[J]. PLOS GENETICS,2015,11(8).
APA Zi, Zhenzhen.,Zhang, Zhuzhen.,Li, Qingrun.,An, Weiwei.,Zeng, Liyong.,...&Wu, Jiarui.(2015).CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse.PLOS GENETICS,11(8).
MLA Zi, Zhenzhen,et al."CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse".PLOS GENETICS 11.8(2015).
Files in This Item: Download All
File Name/Size DocType Version Access License
10.1371@journal.pgen(8691KB)期刊论文出版稿开放获取UnknownView Download
Related Services
Usage statistics
Scholar Google
Similar articles in Scholar Google
[Zi, Zhenzhen]'s Articles
[Zhang, Zhuzhen]'s Articles
[Li, Qingrun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zi, Zhenzhen]'s Articles
[Zhang, Zhuzhen]'s Articles
[Li, Qingrun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zi, Zhenzhen]'s Articles
[Zhang, Zhuzhen]'s Articles
[Li, Qingrun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 10.1371@journal.pgen.1005485.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.