CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse

doi:10.1371/journal.pgen.1005485

	CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse
	Zi, Zhenzhen 1,2; Zhang, Zhuzhen 3; Li, Qingrun 3; An, Weiwei 3; Zeng, Liyong 4; Gao, Dayuan5 ; Yang, Ying 3; Zhu, Xueliang 4; Zeng, Rong3,5 ; Shum, Winnie Waichi5 ; Wu, Jiarui1,2,3,5,6
	2015-08
发表期刊	PLOS GENETICS
ISSN	1553-7404
卷号	11 期号:8
发表状态	已发表
DOI	10.1371/journal.pgen.1005485
摘要	Cyclin Y-like 1 (Ccnyl1) is a newly-identified member of the cyclin family and is highly similar in protein sequences to Cyclin Y (Ccny). However, the function of Ccnyl1 is poorly characterized in any organism. Here we found that Ccnyl1 was most abundantly expressed in the testis of mice and was about seven times higher than the level of Ccny. Male Ccnyl1-/- mice were infertile, whereas both male and female Ccny-/- mice displayed normal fertility. These results suggest that Ccnyl1, but not Ccny, is indispensable for male fertility. Spermatozoa obtained from Ccnyl1-/- mice displayed significantly impaired motility, and represented a thinned annulus region and/or a bent head. We found that the protein, but not the mRNA, level of cyclin-dependent kinase 16 (CDK16) was decreased in the testis of Ccnyl1-/- mice. Further study demonstrated that CCNYL1 interacted with CDK16 and this interaction mutually increased the stability of these two proteins. Moreover, the interaction increased the kinase activity of CDK16. In addition, we observed an alteration of phosphorylation levels of CDK16 in the presence of CCNYL1. We identified the phosphorylation sites of CDK16 by mass spectrometry and revealed that several phosphorylation modifications on the N-terminal region of CDK16 were indispensable for the CCNYL1 binding and the modulation of CDK16 kinase activity. Our results therefore reveal a previously unrecognized role of CCNYL1 in regulating spermatogenesis through the interaction and modulation of CDK16.
收录类别	SCI
语种	英语
资助项目	Science and Technology Commission of Shanghai Municipality[13DZ1940100]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000360823100059
出版者	PUBLIC LIBRARY SCIENCE
WOS关键词	SPERM TERMINAL DIFFERENTIATION ; CELL-CYCLE ; PROTEIN-KINASES ; MAMMALIAN SPERMATOGENESIS ; PHOSPHORYLATION ; ACTIVATION ; PCTAIRE-1 ; FAMILY ; ORGANIZATION ; EXOCYTOSIS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2161
专题	生命科学与技术学院_博士生生命科学与技术学院生命科学与技术学院_PI研究组_岑慧枝组生命科学与技术学院_特聘教授组_曾嵘组
通讯作者	Zi, Zhenzhen
作者单位	1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China 4.Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zi, Zhenzhen,Zhang, Zhuzhen,Li, Qingrun,et al. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse[J]. PLOS GENETICS,2015,11(8).
APA	Zi, Zhenzhen.,Zhang, Zhuzhen.,Li, Qingrun.,An, Weiwei.,Zeng, Liyong.,...&Wu, Jiarui.(2015).CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse.PLOS GENETICS,11(8).
MLA	Zi, Zhenzhen,et al."CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse".PLOS GENETICS 11.8(2015).