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| CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse | |
| 2015-08 | |
| Source Publication | PLOS GENETICS
 |
| ISSN | 1553-7404 |
| Volume | 11Issue:8 |
| Status | 已发表 |
| DOI | 10.1371/journal.pgen.1005485 |
| Abstract | Cyclin Y-like 1 (Ccnyl1) is a newly-identified member of the cyclin family and is highly similar in protein sequences to Cyclin Y (Ccny). However, the function of Ccnyl1 is poorly characterized in any organism. Here we found that Ccnyl1 was most abundantly expressed in the testis of mice and was about seven times higher than the level of Ccny. Male Ccnyl1-/- mice were infertile, whereas both male and female Ccny-/- mice displayed normal fertility. These results suggest that Ccnyl1, but not Ccny, is indispensable for male fertility. Spermatozoa obtained from Ccnyl1-/- mice displayed significantly impaired motility, and represented a thinned annulus region and/or a bent head. We found that the protein, but not the mRNA, level of cyclin-dependent kinase 16 (CDK16) was decreased in the testis of Ccnyl1-/- mice. Further study demonstrated that CCNYL1 interacted with CDK16 and this interaction mutually increased the stability of these two proteins. Moreover, the interaction increased the kinase activity of CDK16. In addition, we observed an alteration of phosphorylation levels of CDK16 in the presence of CCNYL1. We identified the phosphorylation sites of CDK16 by mass spectrometry and revealed that several phosphorylation modifications on the N-terminal region of CDK16 were indispensable for the CCNYL1 binding and the modulation of CDK16 kinase activity. Our results therefore reveal a previously unrecognized role of CCNYL1 in regulating spermatogenesis through the interaction and modulation of CDK16. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Science and Technology Commission of Shanghai Municipality[13DZ1940100] |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:000360823100059 |
| Publisher | PUBLIC LIBRARY SCIENCE |
| WOS Keyword | SPERM TERMINAL DIFFERENTIATION ; CELL-CYCLE ; PROTEIN-KINASES ; MAMMALIAN SPERMATOGENESIS ; PHOSPHORYLATION ; ACTIVATION ; PCTAIRE-1 ; FAMILY ; ORGANIZATION ; EXOCYTOSIS |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2161 |
| Collection | 生命科学与技术学院_博士生 生命科学与技术学院 生命科学与技术学院_PI研究组_岑慧枝组 生命科学与技术学院_特聘教授组_曾嵘组 |
| Corresponding Author | Zi, Zhenzhen |
| Affiliation | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China 4.Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China |
| Recommended Citation GB/T 7714 | Zi, Zhenzhen,Zhang, Zhuzhen,Li, Qingrun,et al. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse[J]. PLOS GENETICS,2015,11(8). |
| APA | Zi, Zhenzhen.,Zhang, Zhuzhen.,Li, Qingrun.,An, Weiwei.,Zeng, Liyong.,...&Wu, Jiarui.(2015).CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse.PLOS GENETICS,11(8). |
| MLA | Zi, Zhenzhen,et al."CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse".PLOS GENETICS 11.8(2015). |
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