Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities

doi:10.3389/fncel.2022.917713

	Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities
	Sheng, Weihao; Zhao, Xueyang; Huang, Xinrui; Yang, Yang
	2022-07-05
发表期刊	FRONTIERS IN CELLULAR NEUROSCIENCE (IF:4.2[JCR-2023],5.1[5-Year])
EISSN	1662-5102
卷号	16
发表状态	已发表
DOI	10.3389/fncel.2022.917713
摘要	The development of in vivo imaging and optogenetic tools makes it possible to control neural circuit activities in an all-optical, closed-loop manner, but such applications are limited by the lack of software for online analysis of neuronal imaging data. We developed an analysis software ORCA (Online Real-time activity and offline Cross-session Analysis), which performs image registration, neuron segmentation, and activity extraction at over 100 frames per second, fast enough to support real-time detection and readout of neural activity. Our active neuron detection algorithm is purely statistical, achieving a much higher speed than previous methods. We demonstrated closed-loop control of neurons that were identified on the fly, without prior recording or image processing. ORCA also includes a cross-session alignment module that efficiently tracks neurons across multiple sessions. In summary, ORCA is a powerful toolbox for fast imaging data analysis and provides a solution for all-optical closed-loop control of neuronal activity.
关键词	calcium imaging closed-loop optogenetic stimulation image registration neuron segmentation fast image processing image alignment
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	ShanghaiTech University["2018YFC1005004","NSFC31970960"]
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000829360200001
出版者	FRONTIERS MEDIA SA
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/214800
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_杨扬组
通讯作者	Yang, Yang
作者单位	ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Sheng, Weihao,Zhao, Xueyang,Huang, Xinrui,et al. Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities[J]. FRONTIERS IN CELLULAR NEUROSCIENCE,2022,16.
APA	Sheng, Weihao,Zhao, Xueyang,Huang, Xinrui,&Yang, Yang.(2022).Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities.FRONTIERS IN CELLULAR NEUROSCIENCE,16.
MLA	Sheng, Weihao,et al."Real-Time Image Processing Toolbox for All-Optical Closed-Loop Control of Neuronal Activities".FRONTIERS IN CELLULAR NEUROSCIENCE 16(2022).