CTP synthase does not form cytoophidia in Drosophila interfollicular stalks

doi:10.1016/j.yexcr.2022.113250

	CTP synthase does not form cytoophidia in Drosophila interfollicular stalks
	Wu, Zheng1 ; Liu, Ji-Long1,2
	2022-09-01
发表期刊	EXPERIMENTAL CELL RESEARCH (IF:3.3[JCR-2023],3.3[5-Year])
ISSN	0014-4827
EISSN	1090-2422
卷号	418 期号:1
发表状态	已发表
DOI	10.1016/j.yexcr.2022.113250
摘要	CTP synthase (CTPS) catalyzes the final step of de novo synthesis of the nucleotide CTP. In 2010, CTPS has been found to form filamentous structures termed cytoophidia in Drosophila follicle cells and germline cells. Subsequently, cytoophidia have been reported in many species across three domains of life: bacteria, eukaryotes and archaea. Forming cytoophidia appears to be a highly conserved and ancient property of CTPS. To our surprise, here we find that polar cells and stalk cells, two specialized types of cells composing Drosophila interfollicular stalks, do not possess obvious cytoophidia. We show that Myc level is low in these two types of cells. Treatment with a glutamine analog, 6-diazo-5-oxo-L-norleucine (DON), increases cytoophidium assembly in main follicle cells, but not in polar cells or stalk cells. Moreover, overexpressing Myc induces cytoophidium formation in stalk cells. When CTPS is overexpressed, cytoophidia can be observed both in stalk cells and polar cells. Our findings provide an interesting paradigm for the in vivo study of cytoophidium assembly and disassembly among different populations of follicle cells.
关键词	CTP synthase Cytoophidium Myc Drosophila Follicle cell Stalk cell Polar cell
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2021YFA0804701-4] ; National Natural Sci-ence Foundation of China[31771490] ; Shanghai Science and Technology Commission[20JC1410500] ; UK Medical Research Council["MC_UU_12021/3","MC_U137788471"]
WOS研究方向	Oncology ; Cell Biology
WOS类目	Oncology ; Cell Biology
WOS记录号	WOS:000833541000001
出版者	ELSEVIER INC
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/214767
专题	生命科学与技术学院_本科生生命科学与技术学院_PI研究组_刘冀珑组
通讯作者	Liu, Ji-Long
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, 230 Haike Rd, Shanghai 201210, Peoples R China 2.Univ Oxford, Dept Physiol Anat & Genet, South Parks Rd, Oxford OX1 3PT, England
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wu, Zheng,Liu, Ji-Long. CTP synthase does not form cytoophidia in Drosophila interfollicular stalks[J]. EXPERIMENTAL CELL RESEARCH,2022,418(1).
APA	Wu, Zheng,&Liu, Ji-Long.(2022).CTP synthase does not form cytoophidia in Drosophila interfollicular stalks.EXPERIMENTAL CELL RESEARCH,418(1).
MLA	Wu, Zheng,et al."CTP synthase does not form cytoophidia in Drosophila interfollicular stalks".EXPERIMENTAL CELL RESEARCH 418.1(2022).