Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion

doi:10.1038/s41590-022-01255-6

	Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
	Chen, Wen 1,2; Chen, Shuangfeng1,3 ; Yan, Chenghua 1; Zhang, Yaguang 1; Zhang, Ronghua 1; Chen, Min 4; Zhong, Shufen 1; Fan, Weiguo 1; Zhu, Songling 1; Zhang, Danyan1,3 ; Lu, Xiao 1; Zhang, Jia 1; Huang, Yuying 1; Zhu, Lin 1; Li, Xuezhen 1; Lv, Dawei 2; Fu, Yadong 1; Iv, Houkun1,3 ; Ling, Zhiyang 1; Ma, Liyan 1; Jiang, Hai 1; Long, Gang 2; Zhu, Jinfang 5; Wu, Dong 4; Wu, Bin 4; Sun, Bing1,3
	2022-07
发表期刊	NATURE IMMUNOLOGY
ISSN	1529-2908
EISSN	1529-2916
发表状态	已发表
DOI	10.1038/s41590-022-01255-6
摘要	["Sun and colleagues describe that the secretion of interleukin-33 is dependent on a p40 N-terminal fragment of gasdermin D, whose generation is independent of inflammatory caspase-1 and caspase-11.","Interleukin-33 (IL-33), an epithelial cell-derived cytokine that responds rapidly to environmental insult, has a critical role in initiating airway inflammatory diseases. However, the molecular mechanism underlying IL-33 secretion following allergen exposure is not clear. Here, we found that two cell events were fundamental for IL-33 secretion after exposure to allergens. First, stress granule assembly activated by allergens licensed the nuclear-cytoplasmic transport of IL-33, but not the secretion of IL-33. Second, a neo-form murine amino-terminal p40 fragment gasdermin D (Gsdmd), whose generation was independent of inflammatory caspase-1 and caspase-11, dominated cytosolic secretion of IL-33 by forming pores in the cell membrane. Either the blockade of stress granule assembly or the abolishment of p40 production through amino acid mutation of residues 309-313 (ELRQQ) could efficiently prevent the release of IL-33 in murine epithelial cells. Our findings indicated that targeting stress granule disassembly and Gsdmd fragmentation could reduce IL-33-dependent allergic airway inflammation."]
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收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2018YFA0507402] ; National Natural Science Foundation of China["81761128009","32000667"] ; Shanghai Science and Technology Innovation Action[21ZR1470600] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2022264] ; Affiliated Hospital of Guangdong Medical University `Clinical Medicine+' CnTech Co-operation Project["CLP2021B001","CLP2021B017"] ; Discipline Construction Project of Guangdong Medical University[4SG21231G] ; Project of Zhanjiang City["2018A01025","2020A01016","2021A05052"] ; Natural Science Foundation of Guangdong Province, China["2021A1515011062","2022A1515011731"]
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000824820700001
出版者	NATURE PORTFOLIO
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/214748
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_孙兵组生命科学与技术学院_硕士生
通讯作者	Zhang, Yaguang; Wu, Dong; Wu, Bin; Sun, Bing
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab C, Shanghai, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci Univ, Inst Pasteur Shanghai, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Guangdong Med Coll, Affiliated Hosp, Inst Resp Dis, Dept Resp & Crit Care Med, Zhanjiang, Peoples R China 5.NIAID, Lab Immune Syst, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Chen, Wen,Chen, Shuangfeng,Yan, Chenghua,et al. Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion[J]. NATURE IMMUNOLOGY,2022.
APA	Chen, Wen.,Chen, Shuangfeng.,Yan, Chenghua.,Zhang, Yaguang.,Zhang, Ronghua.,...&Sun, Bing.(2022).Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion.NATURE IMMUNOLOGY.
MLA	Chen, Wen,et al."Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion".NATURE IMMUNOLOGY (2022).