Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system

doi:10.1038/srep13878

	Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system
	Wang, Xiaolong 1; Yu, Honghao 2,3; Lei, Anmin 4; Zhou, Jiankui5,6 ; Zeng, Wenxian 1; Zhu, Haijing 2,3; Dong, Zhiming 6; Niu, Yiyuan 1; Shi, Bingbo 1; Cai, Bei 1; Liu, Jinwang 2,3; Huang, Shuai 2,3; Yan, Hailong 1,2,3; Zhao, Xiaoe 4; Zhou, Guangxian 1; He, Xiaoling 1; Chen, Xiaoxu 1; Yang, Yuxin 1; Jiang, Yu 1; Shi, Lei 2,3; Tian, Xiue 1; Wang, Yongjun 1; Ma, Baohua 4; Huang, Xingxu5,6 ; Qu, Lei 2,3; Chen, Yulin 1
	2015-09-10
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	5
发表状态	已发表
DOI	10.1038/srep13878
摘要	Recent advances in the study of the CRISPR/Cas9 system have provided a precise and versatile approach for genome editing in various species. However, the applicability and efficiency of this method in large animal models, such as the goat, have not been extensively studied. Here, by co-injection of one-cell stage embryos with Cas9 mRNA and sgRNAs targeting two functional genes (MSTN and FGF5), we successfully produced gene-modified goats with either one or both genes disrupted. The targeting efficiency of MSTN and FGF5 in cultured primary fibroblasts was as high as 60%, while the efficiency of disrupting MSTN and FGF5 in 98 tested animals was 15% and 21% respectively, and 10% for double gene modifications. The on- and off-target mutations of the target genes in fibroblasts, as well as in somatic tissues and testis of founder and dead animals, were carefully analyzed. The results showed that simultaneous editing of several sites was achieved in large animals, demonstrating that the CRISPR/Cas9 system has the potential to become a robust and efficient gene engineering tool in farm animals, and therefore will be critically important and applicable for breeding.
收录类别	SCI
语种	英语
资助项目	Provincial Key Projects of Shaanxi[2014KTDZ02-01]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000360977900001
出版者	NATURE PUBLISHING GROUP
WOS关键词	ONE-STEP GENERATION ; HAIR-GROWTH ; MUTATIONS ; PHENOTYPE ; REGULATOR ; MICE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2137
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Ma, Baohua
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 2.Yulin Univ, Shaanxi Prov Engn & Technol Res Ctr Cashmere Goat, Yulin 719000, Peoples R China 3.Yulin Univ, Life Sci Res Ctr, Yulin 719000, Peoples R China 4.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China 5.Nanjing Univ, Natl Resource Ctr Mutant Mice, MOE Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210061, Jiangsu, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaolong,Yu, Honghao,Lei, Anmin,et al. Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system[J]. SCIENTIFIC REPORTS,2015,5.
APA	Wang, Xiaolong.,Yu, Honghao.,Lei, Anmin.,Zhou, Jiankui.,Zeng, Wenxian.,...&Chen, Yulin.(2015).Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system.SCIENTIFIC REPORTS,5.
MLA	Wang, Xiaolong,et al."Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system".SCIENTIFIC REPORTS 5(2015).