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Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish | |
Shang, Chun-feng1; Li, Xiao-quan1; Yin, Chen1,2; Liu, Bing4,5,6; Wang, Yu-fan1,2; Zhou, Zhuan4,5,6; Du, Jiu-lin1,2,3 | |
2015-11-18 | |
发表期刊 | JOURNAL OF NEUROSCIENCE |
ISSN | 0270-6474 |
卷号 | 35期号:46页码:15291-15294 |
发表状态 | 已发表 |
DOI | 10.1523/JNEUROSCI.3050-15.2015 |
摘要 | Dopamine plays crucial roles in a broad spectrum of brain functions, and neural circuit mechanisms underlying dopaminergic regulation have been intensively studied in the past decade. As larval zebrafish have relatively simple and highly conserved dopaminergic systems, it can serve as an ideal vertebrate animal model to tackle this issue at a whole-brain scale. For this purpose, it is important to develop methods for monitoring endogenous dopamine release in intact larval zebrafish. Here, we developed a real-time method to monitor dopamine release at high spatiotemporal resolution in the brain of awake larval zebrafish using carbon fiber microelectrodes. As an example for application, we combined this method with genetic tools and in vivo calcium imaging and found that food extract can activate pretectal dopaminergic neurons, which in turn release dopamine at the visual center through their projection, providing a dopaminergic circuit mechanism for olfactory modulation of visual functions. Thus, our study demonstrates, for the first time, the utility of carbon fiber microelectrodes for monitoring sensory-evoked dopamine release in the brain of an awake small organism. |
关键词 | carbon fiber electrode cross-modal dopamine electrochemistry pretectum zebrafish |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Science and Technology Commission of Shanghai[14XD1404100] ; Science and Technology Commission of Shanghai[13ZR1464000] |
WOS研究方向 | Neurosciences & Neurology |
WOS类目 | Neurosciences |
WOS记录号 | WOS:000366054500007 |
出版者 | SOC NEUROSCIENCE |
WOS关键词 | STRIATUM IN-VIVO ; NEURONS ; MICROSENSORS ; STIMULI ; LATENCY ; CELLS |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2089 |
专题 | 生命科学与技术学院_特聘教授组_杜久林组 |
通讯作者 | Du, Jiu-lin |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Neurosci,State Key Lab Neurosci, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 4.Peking Univ, State Key Lab Membrane Biol, Inst Mol Med, Beijing 100871, Peoples R China 5.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 6.Peking Univ, PKU IDG McGovern Inst Brain Res, Beijing 100871, Peoples R China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Shang, Chun-feng,Li, Xiao-quan,Yin, Chen,et al. Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish[J]. JOURNAL OF NEUROSCIENCE,2015,35(46):15291-15294. |
APA | Shang, Chun-feng.,Li, Xiao-quan.,Yin, Chen.,Liu, Bing.,Wang, Yu-fan.,...&Du, Jiu-lin.(2015).Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish.JOURNAL OF NEUROSCIENCE,35(46),15291-15294. |
MLA | Shang, Chun-feng,et al."Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish".JOURNAL OF NEUROSCIENCE 35.46(2015):15291-15294. |
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