Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish
Shang, Chun-feng1; Li, Xiao-quan1; Yin, Chen1,2; Liu, Bing4,5,6; Wang, Yu-fan1,2; Zhou, Zhuan4,5,6; Du, Jiu-lin1,2,3
2015-11-18
发表期刊JOURNAL OF NEUROSCIENCE
ISSN0270-6474
卷号35期号:46页码:15291-15294
发表状态已发表
DOI10.1523/JNEUROSCI.3050-15.2015
摘要Dopamine plays crucial roles in a broad spectrum of brain functions, and neural circuit mechanisms underlying dopaminergic regulation have been intensively studied in the past decade. As larval zebrafish have relatively simple and highly conserved dopaminergic systems, it can serve as an ideal vertebrate animal model to tackle this issue at a whole-brain scale. For this purpose, it is important to develop methods for monitoring endogenous dopamine release in intact larval zebrafish. Here, we developed a real-time method to monitor dopamine release at high spatiotemporal resolution in the brain of awake larval zebrafish using carbon fiber microelectrodes. As an example for application, we combined this method with genetic tools and in vivo calcium imaging and found that food extract can activate pretectal dopaminergic neurons, which in turn release dopamine at the visual center through their projection, providing a dopaminergic circuit mechanism for olfactory modulation of visual functions. Thus, our study demonstrates, for the first time, the utility of carbon fiber microelectrodes for monitoring sensory-evoked dopamine release in the brain of an awake small organism.
关键词carbon fiber electrode cross-modal dopamine electrochemistry pretectum zebrafish
收录类别SCI
语种英语
资助项目Science and Technology Commission of Shanghai[14XD1404100] ; Science and Technology Commission of Shanghai[13ZR1464000]
WOS研究方向Neurosciences & Neurology
WOS类目Neurosciences
WOS记录号WOS:000366054500007
出版者SOC NEUROSCIENCE
WOS关键词STRIATUM IN-VIVO ; NEURONS ; MICROSENSORS ; STIMULI ; LATENCY ; CELLS
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2089
专题生命科学与技术学院_特聘教授组_杜久林组
通讯作者Du, Jiu-lin
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Excellence Brain Sci & Intelligence Technol, Inst Neurosci,State Key Lab Neurosci, Shanghai 200031, Peoples R China
2.Univ Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
4.Peking Univ, State Key Lab Membrane Biol, Inst Mol Med, Beijing 100871, Peoples R China
5.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
6.Peking Univ, PKU IDG McGovern Inst Brain Res, Beijing 100871, Peoples R China
通讯作者单位生命科学与技术学院
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GB/T 7714
Shang, Chun-feng,Li, Xiao-quan,Yin, Chen,et al. Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish[J]. JOURNAL OF NEUROSCIENCE,2015,35(46):15291-15294.
APA Shang, Chun-feng.,Li, Xiao-quan.,Yin, Chen.,Liu, Bing.,Wang, Yu-fan.,...&Du, Jiu-lin.(2015).Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish.JOURNAL OF NEUROSCIENCE,35(46),15291-15294.
MLA Shang, Chun-feng,et al."Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish".JOURNAL OF NEUROSCIENCE 35.46(2015):15291-15294.
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