Protein Bricks: 2D and 3D Bio-Nanostructures with Shape and Function on Demand

doi:10.1002/adma.201705919

	Protein Bricks: 2D and 3D Bio-Nanostructures with Shape and Function on Demand
	Jiang, Jianjuan 1; Zhang, Shaoqing 2; Qian, Zhigang 3; Qin, Nan 1; Song, Wenwen 3; Sun, Long 1,4; Zhou, Zhitao 1,4; Shi, Zhifeng 5; Chen, Liang 5; Li, Xinxin1,6 ; Mao, Ying 5; Kaplan, David L.7; Corder, Stephanie N. Gilbert 8; Chen, Xinzhong 8; Liu, Mengkun 8; Omenetto, Fiorenzo G.7; Xia, Xiaoxia 3; Tao, Tiger H.1,2,4,6
	2018-05-17
发表期刊	ADVANCED MATERIALS (IF:27.4[JCR-2023],30.2[5-Year])
ISSN	0935-9648
卷号	30 期号:20
发表状态	已发表
DOI	10.1002/adma.201705919
摘要	Precise patterning of polymer-based biomaterials for functional bio-nanostructures has extensive applications including biosensing, tissue engineering, and regenerative medicine. Remarkable progress is made in both top-down (based on lithographic methods) and bottom-up (via self-assembly) approaches with natural and synthetic biopolymers. However, most methods only yield 2D and pseudo-3D structures with restricted geometries and functionalities. Here, it is reported that precise nanostructuring on genetically engineered spider silk by accurately directing ion and electron beam interactions with the protein's matrix at the nanoscale to create well-defined 2D bionanopatterns and further assemble 3D bionanoarchitectures with shape and function on demand, termed "Protein Bricks." The added control over protein sequence and molecular weight of recombinant spider silk via genetic engineering provides unprecedented lithographic resolution (approaching the molecular limit), sharpness, and biological functions compared to natural proteins. This approach provides a facile method for patterning and immobilizing functional molecules within nanoscopic, hierarchical protein structures, which sheds light on a wide range of biomedical applications such as structure-enhanced fluorescence and biomimetic microenvironments for controlling cell fate.
关键词	biomaterials bio-nanostructures protein Bricks spider silk
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	Shanghai Sailing Program[17YF1422800]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS记录号	WOS:000431961600005
出版者	WILEY-V C H VERLAG GMBH
WOS关键词	ELECTRON-BEAM LITHOGRAPHY ; DNA ORIGAMI ; SILK FIBROIN ; SCAFFOLDS ; FILMS ; NANOSCALE ; HYDROGELS ; POLYMERS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20875
专题	物质科学与技术学院_特聘教授组_陶虎组信息科学与技术学院_特聘教授组_李昕欣组
通讯作者	Xia, Xiaoxia; Tao, Tiger H.
作者单位	1.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China 2.Univ Texas Austin, Dept Mech Engn, Austin, TX 78712 USA 3.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China 4.Univ Chinese Acad Sci, Sch Grad Study, Beijing 100049, Peoples R China 5.Fudan Univ, Huashan Hosp, Dept Neurosurg, Wulumuqi Zhong Rd 12, Shanghai 200040, Peoples R China 6.Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 200031, Peoples R China 7.Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA 8.SUNY Stony Brook, Dept Phys & Astron, Stony Brook, NY 11794 USA
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Jiang, Jianjuan,Zhang, Shaoqing,Qian, Zhigang,et al. Protein Bricks: 2D and 3D Bio-Nanostructures with Shape and Function on Demand[J]. ADVANCED MATERIALS,2018,30(20).
APA	Jiang, Jianjuan.,Zhang, Shaoqing.,Qian, Zhigang.,Qin, Nan.,Song, Wenwen.,...&Tao, Tiger H..(2018).Protein Bricks: 2D and 3D Bio-Nanostructures with Shape and Function on Demand.ADVANCED MATERIALS,30(20).
MLA	Jiang, Jianjuan,et al."Protein Bricks: 2D and 3D Bio-Nanostructures with Shape and Function on Demand".ADVANCED MATERIALS 30.20(2018).