Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure

doi:10.1039/c5sc03705k

	Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure
	Mao, Xiuhai 1,2; Simon, Anna J.3,4; Pei, Hao 1,2; Shi, Jiye 5,6; Li, Jiang 1,2; Huang, Qing 1,2; Plaxco, Kevin W.3,4; Fan, Chunhai1,2,7
	2016
发表期刊	CHEMICAL SCIENCE
ISSN	2041-6520
卷号	7 期号:2 页码:1200-1204
发表状态	已发表
DOI	10.1039/c5sc03705k
摘要	Recognition of the fundamental importance of allosteric regulation in biology dates back to not long after its discovery in the 1960s. Our ability to rationally engineer this potentially useful property into normally non-allosteric catalysts, however, remains limited. In response we report a DNA nanotechnology-enabled approach for introducing allostery into catalytic nucleic acids. Specifically, we have grafted one or two copies of a peroxidase-like DNAzyme, hemin-bound G-quadruplex (hemin-G), onto a DNA tetrahedral nanostructure in such a manner as to cause them to interact, modulating their catalytic activity. We achieve allosteric regulation of these catalysts by incorporating dynamically responsive oligonucleotides that respond to specific "effector" molecules (complementary oligonucleotides or small molecules), altering the spacing between the catalytic sites and thus regulating their activity. This designable approach thus enables subtle allosteric modulation in DNAzymes that is potentially of use for nanomedicine and nanomachines.
收录类别	SCI ; EI
语种	英语
资助项目	Shanghai Municipal Commission for Science and Technology[14ZR1448000]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000368835300045
出版者	ROYAL SOC CHEMISTRY
EI入藏号	20160501876172
EI主题词	Catalysts ; DNA ; DNA sequences ; Medical nanotechnology ; Modulation ; Molecules ; Nanostructures ; Nanotechnology ; Nucleic acids ; Oligonucleotides
EI分类号	Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Nanotechnology:761 ; Chemical Agents and Basic Industrial Chemicals:803 ; Chemical Products Generally:804 ; Atomic and Molecular Physics:931.3
WOS关键词	COMPUTATIONAL DESIGN ; PEROXIDASE-ACTIVITY ; PROTEIN ; ORIGAMI ; ASSEMBLIES ; SWITCHES ; INSIGHTS ; RELEASE ; COMPLEX ; SENSORS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2050
专题	生命科学与技术学院_特聘教授组_樊春海组
通讯作者	Shi, Jiye; Fan, Chunhai
作者单位	1.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Div Phys Biol, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil,Bioimaging Ctr, Shanghai, Peoples R China 3.Univ Calif Santa Barbara, Dept Chem & Biomol Sci, Santa Barbara, CA 93106 USA 4.Univ Calif Santa Barbara, Engn Program, Santa Barbara, CA 93106 USA 5.Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England 6.UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England 7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201200, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Mao, Xiuhai,Simon, Anna J.,Pei, Hao,et al. Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure[J]. CHEMICAL SCIENCE,2016,7(2):1200-1204.
APA	Mao, Xiuhai.,Simon, Anna J..,Pei, Hao.,Shi, Jiye.,Li, Jiang.,...&Fan, Chunhai.(2016).Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.CHEMICAL SCIENCE,7(2),1200-1204.
MLA	Mao, Xiuhai,et al."Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure".CHEMICAL SCIENCE 7.2(2016):1200-1204.