A CRISPR-Based Toolbox for Studying T Cell Signal Transduction

doi:10.1155/2016/5052369

	A CRISPR-Based Toolbox for Studying T Cell Signal Transduction
	Chi, Shen1 ; Weiss, Arthur 2,3; Wang, Haopeng1
	2016
发表期刊	BIOMED RESEARCH INTERNATIONAL (IF:2.6[JCR-2023],3.1[5-Year])
ISSN	2314-6133
发表状态	已发表
DOI	10.1155/2016/5052369
摘要	CRISPR/Cas9 system is a powerful technology to perform genome editing in a variety of cell types. To facilitate the application of Cas9 in mapping T cell signaling pathways, we generated a toolbox for large-scale genetic screens in human Jurkat T cells. The toolbox has three different Jurkat cell lines expressing distinct Cas9 variants, including wild-type Cas9, dCas9-KRAB, and sunCas9. We demonstrated that the toolbox allows us to rapidly disrupt endogenous gene expression at the DNA level and to efficiently repress or activate gene expression at the transcriptional level. The toolbox, in combination with multiple currently existing genome-wide sgRNA libraries, will be useful to systematically investigate T cell signal transduction using both loss-of-function and gain-of-function genetic screens.
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS记录号	WOS:000370326600001
出版者	HINDAWI LTD
WOS关键词	SEQUENCE-SPECIFIC CONTROL ; GENE-EXPRESSION ; GENOME ; RECEPTOR ; ACTIVATION ; REPRESSION ; GENERATION ; KINASES
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2045
专题	生命科学与技术学院_PI研究组_王皞鹏组
通讯作者	Weiss, Arthur; Wang, Haopeng
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 2.Univ Calif San Francisco, Rosalind Russell Med Res Ctr Arthrit, Dept Med, Div Rheumatol, San Francisco, CA 94143 USA 3.Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Chi, Shen,Weiss, Arthur,Wang, Haopeng. A CRISPR-Based Toolbox for Studying T Cell Signal Transduction[J]. BIOMED RESEARCH INTERNATIONAL,2016.
APA	Chi, Shen,Weiss, Arthur,&Wang, Haopeng.(2016).A CRISPR-Based Toolbox for Studying T Cell Signal Transduction.BIOMED RESEARCH INTERNATIONAL.
MLA	Chi, Shen,et al."A CRISPR-Based Toolbox for Studying T Cell Signal Transduction".BIOMED RESEARCH INTERNATIONAL (2016).