Mechanistic insights into staphylopine-mediated metal acquisition

doi:10.1073/pnas.1718382115

	Mechanistic insights into staphylopine-mediated metal acquisition
	Song, Liqiang1 ; Zhang, Yifei1 ; Chen, Weizhong1 ; Gu, Tongnian1 ; Zhang, Shu-Yu 2; Ji, Quanjiang1
	2018-04-10
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
卷号	115 期号:15 页码:3942-3947
发表状态	已发表
DOI	10.1073/pnas.1718382115
摘要	Metal acquisition is vital to pathogens for successful infection within hosts. Staphylopine (StP), a broad-spectrum metallophore biosynthesized by the major human pathogen, Staphylococcus aureus, plays a central role in transition-metal acquisition and bacterial virulence. The StP-like biosynthesis loci are present in various pathogens, and the proteins responsible for StP/metal transportation have been determined. However, the molecular mechanisms of how StP/metal complexes are recognized and transported remain unknown. We report multiple structures of the extracytoplasmic solute-binding protein CntA from the StP/metal transportation system in apo form and in complex with StP and three different metals. We elucidated a sophisticated metal-bound StP recognition mechanism and determined that StP/metal binding triggers a notable interdomain conformational change in CntA. Furthermore, CRISPR/Cas9-mediated single-base substitution mutations and biochemical analysis highlight the importance of StP/metal recognition for StP/metal acquisition. These discoveries provide critical insights into the study of novel metal-acquisition mechanisms in microbes.
关键词	staphylopine metal acquisition CntA CRISPR/Cas9 genome editing
收录类别	SCI ; SCIE
语种	英语
资助项目	China Postdoctoral Science Foundation[2017M620178]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000429540300072
出版者	NATL ACAD SCIENCES
WOS关键词	OLIGOPEPTIDE-BINDING-PROTEIN ; STAPHYLOCOCCUS-AUREUS ; PSEUDOMONAS-AERUGINOSA ; NUTRITIONAL IMMUNITY ; CRYSTAL-STRUCTURES ; ABC-TRANSPORTER ; PEPTIDE BINDING ; NICKEL IMPORT ; IRON ; METALLOPHORE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20251
专题	物质科学与技术学院物质科学与技术学院_PI研究组_季泉江组物质科学与技术学院_硕士生物质科学与技术学院_博士生
通讯作者	Ji, Quanjiang
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Song, Liqiang,Zhang, Yifei,Chen, Weizhong,et al. Mechanistic insights into staphylopine-mediated metal acquisition[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(15):3942-3947.
APA	Song, Liqiang,Zhang, Yifei,Chen, Weizhong,Gu, Tongnian,Zhang, Shu-Yu,&Ji, Quanjiang.(2018).Mechanistic insights into staphylopine-mediated metal acquisition.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(15),3942-3947.
MLA	Song, Liqiang,et al."Mechanistic insights into staphylopine-mediated metal acquisition".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.15(2018):3942-3947.