Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics

doi:10.1016/j.chroma.2022.463273

	Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics
	Liu, Guizhen1,2,4 ; Du, Yanan1,2,4 ; Fu, Tao 1; Han, Ying3 ; Pan, Lifeng 1; Kang, Jingwu 1
	2022-08-02
发表期刊	JOURNAL OF CHROMATOGRAPHY A (IF:3.8[JCR-2023],3.5[5-Year])
ISSN	0021-9673
EISSN	1873-3778
卷号	1676
发表状态	已发表
DOI	10.1016/j.chroma.2022.463273
摘要	An approach for profiling protein-protein interactions by using affinity purification with capillary monolithic immobilized metal affinity chromatography column (cm-IMAC) in combination with label free quantitative proteomics was described in the present work. The cm-IMAC columns were prepared in a single step by copolymerization of the function monomer, namely (S)-2,2′-((1-carboxy-5-(pent4-enamido)pentyl)azanediyl)diacetic acid which provide a nitrilotriacetate (NTA) moiety to form chelated complexation with Ni (II) ions, inside the fused silica capillaries. The His6-tagged bait protein can be easily immobilized on the cm-IMAC columns through the formation of chelating complexation with the NTA-Ni (II) functional groups of the matrix. The cm-IMAC columns were used to explore protein-protein interactions (PPIs) on a proteomic scale when combined with label-free proteomics. A known interaction pair of proteins, namely NDP52 (amino acid sequence 10–126) and NAP1 (33–75) as well as Bcl-2 family proteins were used for proof of concept. New interactors of Bcl-XL were identified and validated by co-immunoprecipitation. © 2022
关键词	Affinity chromatography Chelation Column chromatography Fused silica Molecular biology Nickel compounds Purification Support vector machines Affinity column Affinity purification Capillary monolithic affinity column Chromatography columns Immobilized metal affinity chromatography Label free Monolithics Protein-protein interactions Proteomics Quantitative proteomics
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	National Natural Science Foundations of China[
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
出版者	Elsevier B.V.
EI入藏号	20222612296327
EI主题词	Proteins
EI分类号	461.9 Biology ; 723 Computer Software, Data Handling and Applications ; 801 Chemistry ; 802.2 Chemical Reactions ; 804.1 Organic Compounds ; 812.3 Glass
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/200660
专题	物质科学与技术学院_博士生生命科学与技术学院_公共科研平台_组学分析平台
共同第一作者	Du, Yanan
通讯作者	Kang, Jingwu
作者单位	1.State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai; 200032, China; 2.School of physical science and technology, ShanghaiTech University, Haike Road 100, Shanghai; 200120, China; 3.School of life science and technology, ShanghaiTech University, Haike Road 100, Shanghai; 200120, China; 4.University of Chinese Academy of Sciences, Beijing, China
第一作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Liu, Guizhen,Du, Yanan,Fu, Tao,et al. Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics[J]. JOURNAL OF CHROMATOGRAPHY A,2022,1676.
APA	Liu, Guizhen,Du, Yanan,Fu, Tao,Han, Ying,Pan, Lifeng,&Kang, Jingwu.(2022).Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics.JOURNAL OF CHROMATOGRAPHY A,1676.
MLA	Liu, Guizhen,et al."Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics".JOURNAL OF CHROMATOGRAPHY A 1676(2022).