Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo

doi:10.1016/j.jbc.2022.101965

	Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo
	Wang, Haixiao 1,2; He, Lingjuan 1,3; Li, Yan 1,2; Pu, Wenjuan 1,2; Zhang, Shaohua 1,2; Han, Ximeng 1,2; Lui, Kathy O.4,5; Zhou, Bin1,2,6,7
	2022-06-01
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY (IF:4.0[JCR-2023],4.4[5-Year])
ISSN	0021-9258
EISSN	1083-351X
卷号	298 期号:6
DOI	10.1016/j.jbc.2022.101965
摘要	Genetic technology using site-specific recombinases, such as the Cre-loxP system, has been widely employed for labeling specific cell populations and for studying their functions in vivo. To enhance the precision of cell lineage tracing and functional study, a similar site-specific recombinase system termed Dre-rox has been recently used in combination with Cre-loxP. To enable more specific cell lineage tracing and ablation through dual recombinase activity, we generated two mouse lines that render Dre- or Dre+Cre-mediated recombination to excise a stop codon sequence that prevents the expression of diphtheria toxin receptor (DTR) knocked into the ubiquitously expressed and safe Rosa26 locus. Using different Dre- and Cre-expressing mouse lines, we showed that the surrogate gene reporters tdTomato and DTR were simultaneously expressed in target cells and in their descendants, and we observed efficient ablation of tdTomato+ cells after diphtheria toxin administration. These mouse lines were used to simultaneously trace and deplete the target cells of interest through the inducible expression of a reporter and DTR using dual Cre and Dre recombinases, allowing a more precise and efficient study of the role of specific cell subsets within a heterogeneous population in pathophysiological conditions in vivo. © 2022 THE AUTHORS.
关键词	Cell culture Cell proliferation Cell lineage Cre/LoxP Diphtheria toxin Genetic technology In-vivo Lineage tracing Recombinases Site-specific Target cells Toxin receptors
收录类别	EI
语种	英语
出版者	American Society for Biochemistry and Molecular Biology Inc.
EI入藏号	20222212160509
EI主题词	Ablation
EI分类号	461.9 Biology ; 641.2 Heat Transfer
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/200626
专题	生命科学与技术学院_特聘教授组_周斌组
通讯作者	Zhou, Bin
作者单位	1.State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China; 2.University of Chinese Academy of Sciences, Shanghai, China; 3.School of Life Science, Westlake University, Shanghai, China; 4.Department of Chemical Pathology; 5.Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong; 6.School of Life Science and Technology, ShanghaiTech University, Shanghai, China; 7.School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Haixiao,He, Lingjuan,Li, Yan,et al. Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2022,298(6).
APA	Wang, Haixiao.,He, Lingjuan.,Li, Yan.,Pu, Wenjuan.,Zhang, Shaohua.,...&Zhou, Bin.(2022).Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo.JOURNAL OF BIOLOGICAL CHEMISTRY,298(6).
MLA	Wang, Haixiao,et al."Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo".JOURNAL OF BIOLOGICAL CHEMISTRY 298.6(2022).