Mitochondrial Thioredoxin System as a Modulator of Cyclophilin D Redox State

doi:10.1038/srep23071

	Mitochondrial Thioredoxin System as a Modulator of Cyclophilin D Redox State
	Folda, Alessandra 1; Citta, Anna 1; Scalcon, Valeria 1; Cali, Tito 1; Zonta, Francesco3 ; Scutari, Guido 1; Bindoli, Alberto 2; Rigobello, Maria Pia 1
	2016-03-15
发表期刊	SCIENTIFIC REPORTS (IF:3.8[JCR-2023],4.3[5-Year])
ISSN	2045-2322
卷号	6
发表状态	已发表
DOI	10.1038/srep23071
摘要	The mitochondrial thioredoxin system (NADPH, thioredoxin reductase, thioredoxin) is a major redox regulator. Here we have investigated the redox correlation between this system and the mitochondrial enzyme cyclophilin D. The peptidyl prolyl cis-trans isomerase activity of cyclophilin D was stimulated by the thioredoxin system, while it was decreased by cyclosporin A and the thioredoxin reductase inhibitor auranofin. The redox state of cyclophilin D, thioredoxin 1 and 2 and peroxiredoxin 3 was measured in isolated rat heart mitochondria and in tumor cell lines (CEM-R and HeLa) by redox Western blot analysis upon inhibition of thioredoxin reductase with auranofin, arsenic trioxide, 1-chloro-2,4-dinitrobenzene or after treatment with hydrogen peroxide. A concomitant oxidation of thioredoxin, peroxiredoxin and cyclophilin D was observed, suggesting a redox communication between the thioredoxin system and cyclophilin. This correlation was further confirmed by i) co-immunoprecipitation assay of cyclophilin D with thioredoxin 2 and peroxiredoxin 3, ii) molecular modeling and iii) depleting thioredoxin reductase by siRNA. We conclude that the mitochondrial thioredoxin system controls the redox state of cyclophilin D which, in turn, may act as a regulator of several processes including ROS production and pro-apoptotic factors release.
收录类别	SCI
语种	英语
资助项目	Italian Ministry of University and Research (Bando SIR)[RBSI14C65Z]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000372127200001
出版者	NATURE PUBLISHING GROUP
WOS关键词	PERMEABILITY TRANSITION PORE ; ADENINE-NUCLEOTIDE TRANSLOCASE ; OXIDATIVE STRESS ; CELL-DEATH ; REDUCTASE INHIBITORS ; HEART-MITOCHONDRIA ; ISOMERASE ACTIVITY ; ANTICANCER AGENTS ; CARBENE COMPLEXES ; PHOSPHATE CARRIER
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1896
专题	免疫化学研究所_特聘教授组_功能筛选实验室免疫化学研究所_特聘教授组_Michael Levitt组
通讯作者	Bindoli, Alberto; Rigobello, Maria Pia
作者单位	1.Univ Padua, Dept Biomed Sci, Via Ugo Bassi 58-B, I-35131 Padua, Italy 2.CNR, Inst Neurosci, Viale G Colombo 3, I-35131 Padua, Italy 3.ShanghaiTech Univ, SIAIS, 99 Haike Rd, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Folda, Alessandra,Citta, Anna,Scalcon, Valeria,et al. Mitochondrial Thioredoxin System as a Modulator of Cyclophilin D Redox State[J]. SCIENTIFIC REPORTS,2016,6.
APA	Folda, Alessandra.,Citta, Anna.,Scalcon, Valeria.,Cali, Tito.,Zonta, Francesco.,...&Rigobello, Maria Pia.(2016).Mitochondrial Thioredoxin System as a Modulator of Cyclophilin D Redox State.SCIENTIFIC REPORTS,6.
MLA	Folda, Alessandra,et al."Mitochondrial Thioredoxin System as a Modulator of Cyclophilin D Redox State".SCIENTIFIC REPORTS 6(2016).