Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo

doi:10.1016/j.bbrc.2016.02.062

	Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo
	Liu, Qiaozhen 1; Zhang, Hui 1; Tian, Xueying 1; He, Lingjuan 1; Huang, Xiuzhen 1; Tan, Zhen 2; Yan, Yan 3; Evans, Sylvia M.4; Wythe, Joshua D.5; Zhou, Bin1,6,7
	2016-03-18
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	471 期号:4 页码:430-436
发表状态	已发表
DOI	10.1016/j.bbrc.2016.02.062
摘要	Recent identification of the neonatal 2nd coronary vascular population (2nd CVP) suggests that a subset of these vessels form de novo and mature in the inner myocardial wall of the postnatal heart. However, the origin of smooth muscle cells (SMCs) in the postnatal 2nd CVP remains undetermined. Using a tamoxifen-inducible Wt1-CreER driver and a Rosa26-RFP reporter line, we traced the lineage of epicardial cells to determine if they contribute to SMCs of the 2nd CVP: Late embryonic and postnatal induction of Wt1-CreER activity demonstrated that at these stages Wt1-labeled epicardium does not significantly migrate into the myocardium to form SMCs. However, following tamoxifen treatment at an early embryonic stage (E10.5), we detected Wt1 descendants (epicardium-derived cells, or EPDCs) in the outer myocardial wall at E17.5. When the 2nd CVP forms and remodels at postnatal stage, these early labeled EDPCs re-migrate deep into the inner myocardial wall and contribute to 2nd CVP-SMCs in the adult heart. Our findings reveal that SMCs in the postnatal 2nd CVP are pre-specified as EPDCs from the earliest wave of epicardial cell migration. Rather than the re-activation and migration of epicardial cells at later stages, these resident EPDCs mobilize and contribute to smooth muscle of the 2nd CVP during postnatal development. (C) 2016 Elsevier Inc. All rights reserved.
关键词	Lineage tracing Epicardium Epicardium-derived cells (EPDCs) Smooth muscle 2nd coronary vascular population (2nd CVP)
收录类别	SCI
语种	英语
资助项目	American Heart Association[12SDG12060353]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000373242600007
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	EPICARDIUM-DERIVED CELLS ; CORONARY-ARTERIES ; ANGIOGENESIS ; LINEAGE ; ADULT ; FATE ; FORM ; POPULATION ; CONTRIBUTE ; DIRECTS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1893
专题	生命科学与技术学院_特聘教授组_周斌组
通讯作者	Zhou, Bin
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Grad Sch, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Dept Pediat Hematol Oncol, Xinhua Hosp, Shanghai 200092, Peoples R China 3.Fudan Univ, Zhongshan Hosp, Shanghai 200032, Peoples R China 4.Univ Calif San Diego, Dept Pharmacol, Dept Med, Skaggs Sch Pharm, La Jolla, CA 92093 USA 5.Baylor Coll Med, Dept Mol Physiol & Biophys, Cardiovasc Res Inst, Houston, TX 77030 USA 6.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci,CAS Ctr Excellence Brain S, Shanghai 200031, Peoples R China 7.ShanghaiTech Univ, Shanghai 201210, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Liu, Qiaozhen,Zhang, Hui,Tian, Xueying,et al. Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,471(4):430-436.
APA	Liu, Qiaozhen.,Zhang, Hui.,Tian, Xueying.,He, Lingjuan.,Huang, Xiuzhen.,...&Zhou, Bin.(2016).Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,471(4),430-436.
MLA	Liu, Qiaozhen,et al."Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 471.4(2016):430-436.