Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo
Liu, Qiaozhen1; Zhang, Hui1; Tian, Xueying1; He, Lingjuan1; Huang, Xiuzhen1; Tan, Zhen2; Yan, Yan3; Evans, Sylvia M.4; Wythe, Joshua D.5; Zhou, Bin1,6,7
2016-03-18
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume471Issue:4Pages:430-436
Status已发表
DOI10.1016/j.bbrc.2016.02.062
AbstractRecent identification of the neonatal 2nd coronary vascular population (2nd CVP) suggests that a subset of these vessels form de novo and mature in the inner myocardial wall of the postnatal heart. However, the origin of smooth muscle cells (SMCs) in the postnatal 2nd CVP remains undetermined. Using a tamoxifen-inducible Wt1-CreER driver and a Rosa26-RFP reporter line, we traced the lineage of epicardial cells to determine if they contribute to SMCs of the 2nd CVP: Late embryonic and postnatal induction of Wt1-CreER activity demonstrated that at these stages Wt1-labeled epicardium does not significantly migrate into the myocardium to form SMCs. However, following tamoxifen treatment at an early embryonic stage (E10.5), we detected Wt1 descendants (epicardium-derived cells, or EPDCs) in the outer myocardial wall at E17.5. When the 2nd CVP forms and remodels at postnatal stage, these early labeled EDPCs re-migrate deep into the inner myocardial wall and contribute to 2nd CVP-SMCs in the adult heart. Our findings reveal that SMCs in the postnatal 2nd CVP are pre-specified as EPDCs from the earliest wave of epicardial cell migration. Rather than the re-activation and migration of epicardial cells at later stages, these resident EPDCs mobilize and contribute to smooth muscle of the 2nd CVP during postnatal development. (C) 2016 Elsevier Inc. All rights reserved.
KeywordLineage tracing Epicardium Epicardium-derived cells (EPDCs) Smooth muscle 2nd coronary vascular population (2nd CVP)
Indexed BySCI
Language英语
Funding ProjectAmerican Heart Association[12SDG12060353]
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000373242600007
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
WOS KeywordEPICARDIUM-DERIVED CELLS ; CORONARY-ARTERIES ; ANGIOGENESIS ; LINEAGE ; ADULT ; FATE ; FORM ; POPULATION ; CONTRIBUTE ; DIRECTS
Original Document TypeArticle
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1893
Collection生命科学与技术学院_特聘教授组_周斌组
Corresponding AuthorZhou, Bin
Affiliation1.Chinese Acad Sci, Shanghai Inst Biol Sci, Grad Sch, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Med, Dept Pediat Hematol Oncol, Xinhua Hosp, Shanghai 200092, Peoples R China
3.Fudan Univ, Zhongshan Hosp, Shanghai 200032, Peoples R China
4.Univ Calif San Diego, Dept Pharmacol, Dept Med, Skaggs Sch Pharm, La Jolla, CA 92093 USA
5.Baylor Coll Med, Dept Mol Physiol & Biophys, Cardiovasc Res Inst, Houston, TX 77030 USA
6.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci,CAS Ctr Excellence Brain S, Shanghai 200031, Peoples R China
7.ShanghaiTech Univ, Shanghai 201210, Peoples R China
Corresponding Author AffilicationShanghaiTech University
Recommended Citation
GB/T 7714
Liu, Qiaozhen,Zhang, Hui,Tian, Xueying,et al. Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,471(4):430-436.
APA Liu, Qiaozhen.,Zhang, Hui.,Tian, Xueying.,He, Lingjuan.,Huang, Xiuzhen.,...&Zhou, Bin.(2016).Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,471(4),430-436.
MLA Liu, Qiaozhen,et al."Smooth muscle origin of postnatal 2nd CVP is pre-determined in early embryo".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 471.4(2016):430-436.
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