ShanghaiTech University Knowledge Management System
| Synergistic action of master transcription factors controls epithelial-to-mesenchymal transition | |
| 2016-04-07 | |
| Source Publication | NUCLEIC ACIDS RESEARCH
 |
| ISSN | 0305-1048 |
| Volume | 44Issue:6Pages:2514-2527 |
| Status | 已发表 |
| DOI | 10.1093/nar/gkw126 |
| Abstract | Epithelial-to-mesenchymal transition (EMT) is a complex multistep process in which phenotype switches are mediated by a network of transcription factors (TFs). Systematic characterization of all dynamic TFs controlling EMT state transitions, especially for the intermediate partial-EMT state, represents a highly relevant yet largely unexplored task. Here, we performed a computational analysis that integrated time-course EMT transcriptomic data with public cistromic data and identified three synergistic master TFs (ETS2, HNF4A and JUNB) that regulate the transition through the partial-EMT state. Overexpression of these regulators predicted a poor clinical outcome, and their elimination readily abolished TGF-beta-induced EMT. Importantly, these factors utilized a clique motif, physically interact and their cumulative binding generally characterized EMT-associated genes. Furthermore, analyses of H3K27ac ChIP-seq data revealed that ETS2, HNF4A and JUNB are associated with super-enhancers and the administration of BRD4 inhibitor readily abolished TGF-beta-induced EMT. These findings have implications for systematic discovery of master EMT regulators and super-enhancers as novel targets for controlling metastasis. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Science and Technology Commission of Shanghai Municipality[12DZ1910800] |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| WOS ID | WOS:000374570500014 |
| Publisher | OXFORD UNIV PRESS |
| WOS Keyword | TGF-BETA ; SUPER-ENHANCERS ; REGULATORY NETWORKS ; CELL-MIGRATION ; CANCER ; EXPRESSION ; PROGRESSION ; METASTASIS ; INHIBITION ; ACTIVATION |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1874 |
| Collection | 生命科学与技术学院 生命科学与技术学院_特聘教授组_王鹏组 生命科学与技术学院_硕士生 |
| Corresponding Author | Wang, Peng |
| Affiliation | 1.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Syst Biol, 100 Haike Rd,Zhangjiang High Tech Pk, Shanghai 201210, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China 4.Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zu Chong Zhi Rd,Zhangjiang High Tech Pk, Shanghai 201203, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd,Zhangjiang High Tech Pk, Shanghai 201210, Peoples R China |
| Corresponding Author Affilication | School of Life Science and Technology |
| Recommended Citation GB/T 7714 | Chang, Hongyuan,Liu, Yuwei,Xue, Mengzhu,et al. Synergistic action of master transcription factors controls epithelial-to-mesenchymal transition[J]. NUCLEIC ACIDS RESEARCH,2016,44(6):2514-2527. |
| APA | Chang, Hongyuan.,Liu, Yuwei.,Xue, Mengzhu.,Liu, Haiyue.,Du, Shaowei.,...&Wang, Peng.(2016).Synergistic action of master transcription factors controls epithelial-to-mesenchymal transition.NUCLEIC ACIDS RESEARCH,44(6),2514-2527. |
| MLA | Chang, Hongyuan,et al."Synergistic action of master transcription factors controls epithelial-to-mesenchymal transition".NUCLEIC ACIDS RESEARCH 44.6(2016):2514-2527. |
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