Importin 1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal

doi:10.1074/jbc.M115.700823

	Importin 1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal
	Wang, Shimin 1; Lu, Yi 1; Yin, Meng-Xin 1; Wang, Chao 1; Wu, Wei 1; Li, Jinhui 1; Wu, Wenqing 1; Ge, Ling 1; Hu, Lianxin 1; Zhao, Yun1,2 ; Zhang, Lei1,2
	2016-04-08
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY (IF:4.0[JCR-2023],4.4[5-Year])
ISSN	0021-9258
卷号	291 期号:15 页码:7926-7937
发表状态	已发表
DOI	10.1074/jbc.M115.700823
摘要	The Hippo signaling pathway controls organ size by orchestrating cell proliferation and apoptosis. When the Hippo pathway was inactivated, the transcriptional co-activator Yorkie translocates into the nucleus and forms a complex with transcription factor Scalloped to promote the expression of Hippo pathway target genes. Therefore, the nuclear translocation of Yorkie is a critical step in Hippo signaling. Here, we provide evidence that the N-terminal 1-55 amino acids of Yorkie, especially Arg-15, were essential for its nuclear localization. By mass spectrometry and biochemical analyses, we found that Importin 1 can directly interact with the Yorkie N terminus and drive Yorkie into the nucleus. Further experiments show that the upstream component Hippo can inhibit Importin 1-mediated Yorkie nuclear import. Taken together, we identified a potential nuclear localization signal at the N-terminal end of Yorkie as well as a critical role for Importin 1 in Yorkie nuclear import.
关键词	Hippo pathway nuclear translocation nuclear transport oncogene yes-associated protein (YAP) Importin NLS yorkie
收录类别	SCI ; EI
语种	英语
资助项目	State Key Program of National Natural Science of China[31530043]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000374056700015
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
EI入藏号	20161802340484
EI主题词	Cell death ; Cell proliferation ; Gene expression ; Mass spectrometry ; Transcription
EI分类号	Biomedical Engineering:461.1 ; Biology:461.9 ; Chemistry:801
WOS关键词	ORGAN SIZE CONTROL ; TUMOR-SUPPRESSOR PATHWAY ; CELL-PROLIFERATION ; PROMOTES APOPTOSIS ; CRYSTALLOGRAPHIC ANALYSIS ; KARYOPHERIN ALPHA ; STRUCTURAL BASIS ; TEAD/TEF FAMILY ; BANTAM MICRORNA ; PROTEIN IMPORT
原始文献类型	Article
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1872
专题	生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_张雷组
通讯作者	Zhang, Lei
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Cell Biol,Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,Innovat Ctr Cell, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Shimin,Lu, Yi,Yin, Meng-Xin,et al. Importin 1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(15):7926-7937.
APA	Wang, Shimin.,Lu, Yi.,Yin, Meng-Xin.,Wang, Chao.,Wu, Wei.,...&Zhang, Lei.(2016).Importin 1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal.JOURNAL OF BIOLOGICAL CHEMISTRY,291(15),7926-7937.
MLA	Wang, Shimin,et al."Importin 1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal".JOURNAL OF BIOLOGICAL CHEMISTRY 291.15(2016):7926-7937.