Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm

doi:10.1107/S2052252521012963

KMS > 大科学中心

	Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm
	Pan, Dan1,2 ; Fan, Jiadong1,2 ; Nie, Zhenzhen 3,4; Sun, Zhibin1,2,5 ; Zhang, Jianhua1,2 ; Tong, Yajun1,2 ; He, Bo1,2 ; Song, Changyong 6; Kohmura, Yoshiki 7; Yabashi, Makina 7; Ishikawa, Tetsuya 7; Shen, Yuequan 3,4; Jiang, Huaidong1,2
	2022-03
发表期刊	IUCRJ
ISSN	2052-2525
卷号	9
发表状态	已发表
DOI	10.1107/S2052252521012963
摘要	Radiation damage and a low signal-to-noise ratio are the primary factors that limit spatial resolution in coherent diffraction imaging (CDI) of biomaterials using X-ray sources. Introduced here is a clustering algorithm named ConvRe based on deep learning, and it is applied to obtain accurate and consistent image reconstruction from noisy diffraction patterns of weakly scattering biomaterials. To investigate the impact of X-ray radiation on soft biomaterials, CDI experiments were performed on mitochondria from human embryonic kidney cells using synchrotron radiation. Benefiting from the new algorithm, structural changes in the mitochondria induced by X-ray radiation damage were quantitatively characterized and analysed at the nanoscale with different radiation doses. This work also provides a promising approach for improving the imaging quality of biomaterials with XFEL-based plane-wave CDI.
关键词	coherent diffraction imaging radiation damage mitochondria clustering algorithms X-ray imaging quantitative analysis
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Major State Basic Research Development Program of China[2017YFA0504802] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB 37040303] ; National Natural Science Foundation of China[21727817]
WOS研究方向	Chemistry ; Crystallography ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary
WOS记录号	WOS:000795673400009
出版者	INT UNION CRYSTALLOGRAPHY
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/185358
专题	大科学中心大科学中心_PI研究组_江怀东组硬x射线自由电子激光装置项目物质科学与技术学院_硕士生物质科学与技术学院_博士生
通讯作者	Fan, Jiadong; Jiang, Huaidong
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Ctr Transformat Sci, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 3.Nankai Univ, State Key Lab Med Chem Biol, 94 Weijin Rd, Tianjin 300071, Peoples R China 4.Nankai Univ, Coll Life Sci, 94 Weijin Rd, Tianjin 300071, Peoples R China 5.Paul Scherrer Inst, Photon Sci Div, CH-5232 Villigen, Switzerland 6.Pohang Univ Sci & Technol, Dept Phys, Pohang 37673, South Korea 7.RIKEN, SPring Ctr 8, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan
第一作者单位	物质科学与技术学院; 上海科技大学
通讯作者单位	物质科学与技术学院; 上海科技大学
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Pan, Dan,Fan, Jiadong,Nie, Zhenzhen,et al. Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm[J]. IUCRJ,2022,9.
APA	Pan, Dan.,Fan, Jiadong.,Nie, Zhenzhen.,Sun, Zhibin.,Zhang, Jianhua.,...&Jiang, Huaidong.(2022).Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm.IUCRJ,9.
MLA	Pan, Dan,et al."Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm".IUCRJ 9(2022).