ShanghaiTech University Knowledge Management System
| Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm | |
| 2022-03 | |
| Source Publication | IUCRJ
 |
| ISSN | 2052-2525 |
| Volume | 9 |
| Status | 已发表 |
| DOI | 10.1107/S2052252521012963 |
| Abstract | Radiation damage and a low signal-to-noise ratio are the primary factors that limit spatial resolution in coherent diffraction imaging (CDI) of biomaterials using X-ray sources. Introduced here is a clustering algorithm named ConvRe based on deep learning, and it is applied to obtain accurate and consistent image reconstruction from noisy diffraction patterns of weakly scattering biomaterials. To investigate the impact of X-ray radiation on soft biomaterials, CDI experiments were performed on mitochondria from human embryonic kidney cells using synchrotron radiation. Benefiting from the new algorithm, structural changes in the mitochondria induced by X-ray radiation damage were quantitatively characterized and analysed at the nanoscale with different radiation doses. This work also provides a promising approach for improving the imaging quality of biomaterials with XFEL-based plane-wave CDI. |
| Keyword | coherent diffraction imaging radiation damage mitochondria clustering algorithms X-ray imaging quantitative analysis |
| URL | 查看原文 |
| Indexed By | SCI ; SCIE |
| Language | 英语 |
| Funding Project | Major State Basic Research Development Program of China[2017YFA0504802] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB 37040303] ; National Natural Science Foundation of China[21727817] |
| WOS Research Area | Chemistry ; Crystallography ; Materials Science |
| WOS Subject | Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary |
| WOS ID | WOS:000795673400009 |
| Publisher | INT UNION CRYSTALLOGRAPHY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/185358 |
| Collection | 大科学中心 大科学中心_PI研究组_江怀东组 硬x射线自由电子激光装置项目 物质科学与技术学院_硕士生 物质科学与技术学院_博士生 |
| Corresponding Author | Fan, Jiadong; Jiang, Huaidong |
| Affiliation | 1.ShanghaiTech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Ctr Transformat Sci, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 3.Nankai Univ, State Key Lab Med Chem Biol, 94 Weijin Rd, Tianjin 300071, Peoples R China 4.Nankai Univ, Coll Life Sci, 94 Weijin Rd, Tianjin 300071, Peoples R China 5.Paul Scherrer Inst, Photon Sci Div, CH-5232 Villigen, Switzerland 6.Pohang Univ Sci & Technol, Dept Phys, Pohang 37673, South Korea 7.RIKEN, SPring Ctr 8, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan |
| First Author Affilication | School of Physical Science and Technology; ShanghaiTech University |
| Corresponding Author Affilication | School of Physical Science and Technology; ShanghaiTech University |
| First Signature Affilication | School of Physical Science and Technology |
| Recommended Citation GB/T 7714 | Pan, Dan,Fan, Jiadong,Nie, Zhenzhen,et al. Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm[J]. IUCRJ,2022,9. |
| APA | Pan, Dan.,Fan, Jiadong.,Nie, Zhenzhen.,Sun, Zhibin.,Zhang, Jianhua.,...&Jiang, Huaidong.(2022).Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm.IUCRJ,9. |
| MLA | Pan, Dan,et al."Quantitative analysis of the effect of radiation on mitochondria structure using coherent diffraction imaging with a clustering algorithm".IUCRJ 9(2022). |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
Edit Comment
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.