SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis

doi:10.1242/dev.130773

	SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis
	Lv, Xiangdong 1; Pan, Chenyu 1; Zhang, Zhao 1; Xia, Yuanxin 1; Chen, Hao 1; Zhang, Shuo 1; Guo, Tong 1; Han, Hui 1; Song, Haiyun 2; Zhang, Lei1,3 ; Zhao, Yun1,3
	2016-05-15
发表期刊	DEVELOPMENT
ISSN	0950-1991
卷号	143 期号:10 页码:1655-1662
发表状态	已发表
DOI	10.1242/dev.130773
摘要	SUMO (Small ubiquitin-related modifier) modification (SUMOylation) is a highly dynamic post-translational modification (PTM) that plays important roles in tissue development and disease progression. However, its function in adult stem cell maintenance is largely unknown. Here, we report the function of SUMOylation in somatic cyst stem cell (CySC) self-renewal in adult Drosophila testis. The SUMO pathway cell-autonomously regulates CySC maintenance. Reduction of SUMOylation promotes premature differentiation of CySCs and impedes the proliferation of CySCs, which leads to a reduction in the number of CySCs. Consistent with this, CySC clones carrying a mutation of the SUMO-conjugating enzyme are rapidly lost. Furthermore, inhibition of the SUMO pathway phenocopies disruption of the Hedgehog (Hh) pathway, and can block the proliferation of CySCs induced by Hh activation. Importantly, the SUMO pathway directly regulates the SUMOylation of Hh pathway transcription factor Cubitus interruptus (Ci), which is required for promoting CySC proliferation. Thus, we conclude that SUMO directly targets the Hh pathway and regulates CySC maintenance in adult Drosophila testis.
关键词	SUMOylation Hedgehog Adult stem cell Testis Drosophila
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31171414] ; National Natural Science Foundation of China[31371492]
WOS研究方向	Developmental Biology
WOS类目	Developmental Biology
WOS记录号	WOS:000389588300006
出版者	COMPANY OF BIOLOGISTS LTD
WOS关键词	NUCLEAR-PORE COMPLEX ; SELF-RENEWAL ; ESCHERICHIA-COLI ; GERMLINE ; NICHE ; PROTEIN ; SUMOYLATION ; ROLES ; DISEASE ; HH
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1833
专题	生命科学与技术学院_特聘教授组_赵允组生命科学与技术学院_特聘教授组_张雷组
通讯作者	Zhao, Yun
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Cell Biol,Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,Innovat Ctr Cell, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Key Lab Food Safety Res, Inst Nutr Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Lv, Xiangdong,Pan, Chenyu,Zhang, Zhao,et al. SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis[J]. DEVELOPMENT,2016,143(10):1655-1662.
APA	Lv, Xiangdong.,Pan, Chenyu.,Zhang, Zhao.,Xia, Yuanxin.,Chen, Hao.,...&Zhao, Yun.(2016).SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis.DEVELOPMENT,143(10),1655-1662.
MLA	Lv, Xiangdong,et al."SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis".DEVELOPMENT 143.10(2016):1655-1662.