Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells

doi:10.1107/S2052252517017912

	Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells
	Yao, Shengkun1,2 ; Fan, Jiadong1 ; Chen, Zhiyun 3; Zong, Yunbing 4; Zhang, Jianhua1,4 ; Sun, Zhibin1,4 ; Zhang, Lijuan 5; Tai, Renzhong 5; Liu, Zhi1 ; Chen, Chunying 3; Jiang, Huaidong1,2,4
	2018-03
发表期刊	IUCRJ (IF:2.9[JCR-2023],3.3[5-Year])
ISSN	2052-2525
卷号	5 页码:141-149
发表状态	已发表
DOI	10.1107/S2052252517017912
摘要	Knowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography ( EST). Macrophages with a size of similar to 20 mu m that had been exposed to the potential antitumour agent [Gd@C-82(OH)(22)](n) were investigated. Large numbers of nanoparticles (NPs) aggregated within the cell and were mainly located in phagosomes. No NPs were observed in the nucleus. Imaging of the nanomedicine within whole cells advanced the understanding of the highefficiency antitumour activity and the low toxicity of this agent. This imaging technique can be used to probe nanomaterials within intact large-size cells at nanometre resolution uniformly in three dimensions and may greatly benefit the fields of nanomedicine and nanotoxicology.
关键词	cellular imaging X-ray tomography equally sloped tomography dual-energy contrast computed tomography nanoscience X-ray microscopy imaging structure determination
收录类别	SCI ; SCIE
语种	英语
资助项目	Major State Basic Research Development Program of China[2014CB910401]
WOS研究方向	Chemistry ; Crystallography ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary
WOS记录号	WOS:000427041400006
出版者	INT UNION CRYSTALLOGRAPHY
WOS关键词	X-RAY TOMOGRAPHY ; FLUORESCENCE MICROSCOPY ; TUMOR MICROENVIRONMENT ; FULLERENE DERIVATIVES ; ELECTRON TOMOGRAPHY ; NANOPARTICLES ; ABSORPTION ; 3D ; MACROPHAGE ; RESOLUTION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/18302
专题	物质科学与技术学院大科学中心_PI研究组_刘志组大科学中心_PI研究组_江怀东组 iHuman研究所硬x射线自由电子激光装置项目
通讯作者	Jiang, Huaidong
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, IHuman Inst, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 3.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, 11 ZhongGuanCun BeiYiTiao, Beijing 100190, Peoples R China 4.Shandong Univ, State Key Lab Crystal Mat, 27 Shanda Nanlu, Jinan 250100, Shandong, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, 239 Zhangheng Rd, Shanghai 201204, Peoples R China
第一作者单位	物质科学与技术学院; iHuman研究所
通讯作者单位	物质科学与技术学院; iHuman研究所
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Yao, Shengkun,Fan, Jiadong,Chen, Zhiyun,et al. Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells[J]. IUCRJ,2018,5:141-149.
APA	Yao, Shengkun.,Fan, Jiadong.,Chen, Zhiyun.,Zong, Yunbing.,Zhang, Jianhua.,...&Jiang, Huaidong.(2018).Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.IUCRJ,5,141-149.
MLA	Yao, Shengkun,et al."Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells".IUCRJ 5(2018):141-149.