Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL

doi:10.1073/pnas.1716439115

	Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL
	Wu, Yanfang 1,2; Li, Xia 1,2; Jia, Junying 3; Zhang, Yanpeng4 ; Li, Jing 5; Zhu, Zhengmao 5; Wang, Huaqing 6; Tang, Jie 1; Hu, Junjie 1,2
	2018-03-20
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	115 期号:12 页码:E2762-E2771
发表状态	已发表
DOI	10.1073/pnas.1716439115
摘要	The accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and triggers the unfolded protein response (UPR). Failure to resolve ER stress leads to apoptotic cell death via a yet unclear mechanism. Here, we show that RNF183, a membrane-spanning RING finger protein, localizes to the ER and exhibits classic E3 ligase activities. Sustained ER stress induced by different treatments increases RNF183 protein levels posttranscriptionally in an IRE1 alpha-dependent manner. Activated IRE1 reduces the level of miR-7, which increases the stability of RNF183 transcripts. In addition, overexpression of RNF183 leads to increased apoptosis and its depletion alleviates ER stress-induced apoptosis. Furthermore, RNF183 interacts with Bcl-xL, an antiapoptotic member of the Bcl-2 family, and polyubiquitinates Bcl-xL for degradation. Thus, RNF183 plays an important role in executing programmed cell death upon prolonged ER stress, likely by inducing apoptosis through Bcl-xL.
关键词	RING finger protein endoplasmic reticulum stress unfolded protein response apoptosis Bcl-xL
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31225006] ; National Natural Science Foundation of China[3142100024]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000427829500015
出版者	NATL ACAD SCIENCES
WOS关键词	UNFOLDED-PROTEIN-RESPONSE ; ENDOPLASMIC-RETICULUM STRESS ; PROGRAMMED CELL-DEATH ; TRANSCRIPTION FACTOR ; FUNCTIONAL FEATURES ; MESSENGER-RNA ; IRE1 ; DEGRADATION ; MITOCHONDRIA ; TRANSLATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/18278
专题	生命科学与技术学院生命科学与技术学院_硕士生
通讯作者	Hu, Junjie
作者单位	1.Chinese Acad Sci, Inst Biophys, Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ CAS, Coll Life Sci, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Prot Sci Core Facil Ctr, Beijing 100101, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 5.Nankai Univ, Coll Life Sci, Dept Genet & Cell Biol, Tianjin 300071, Peoples R China 6.Tianjin Union Med Ctr, Inst Integrat Oncol, Dept Oncol, Tianjin 300121, Peoples R China
推荐引用方式 GB/T 7714	Wu, Yanfang,Li, Xia,Jia, Junying,et al. Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(12):E2762-E2771.
APA	Wu, Yanfang.,Li, Xia.,Jia, Junying.,Zhang, Yanpeng.,Li, Jing.,...&Hu, Junjie.(2018).Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(12),E2762-E2771.
MLA	Wu, Yanfang,et al."Transmembrane E3 ligase RNF183 mediates ER stress-induced apoptosis by degrading Bcl-xL".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.12(2018):E2762-E2771.