上海科技大学知识管理系统

Provided are compositions and methods for enhanced prime editing, which include a pegRNA that encodes a target mutation in a target protein, along with one or more nearby silent or conservative mutations. These silent mutations can increase the editing efficiency, without causing a change to the target protein sequence. Also provided are compositions and methods of using the improved prime editing for preventing or treating infections by SARS-CoV or SARS-CoV-2.

本发明提供了用于增强原始编辑的组合物和方法，其包括编码靶蛋白中的靶突变以及一个或多个附近沉默或保守突变的pEGRNA。 这些沉默突变可以提高编辑效率，而不引起靶蛋白序列的改变。 还提供了使用改进的原编辑预防或治疗SARS-CoV或SARS-CoV-2感染的组合物和方法。