Unique binding pattern for a lineage of human antibodies with broad reactivity against influenza A virus

doi:10.1038/s41467-022-29950-w

	Unique binding pattern for a lineage of human antibodies with broad reactivity against influenza A virus
	Sun, Xiaoyu 1; Liu, Caixuan 2; Lu, Xiao 1; Ling, Zhiyang 1; Yi, Chunyan 1; Zhang, Zhen 1; Li, Zi 3; Jin, Mingliang 2; Wang, Wenshuai 1; Tang, Shubing 4; Wang, Fangfang 5; Wang, Fang 1; Wangmo, Sonam1,6 ; Chen, Shuangfeng1,6 ; Li, Li6 ; Ma, Liyan 1; Zhang, Yaguang 1; Yang, Zhuo 1; Dong, Xiaoping 7; Qian, Zhikang 8; Ding, Jianping 1; Wang, Dayan 3; Cong, Yao 2; Sun, Bing1,6
	2022-05-02
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	13 期号:1
发表状态	已发表
DOI	10.1038/s41467-022-29950-w
摘要	["While most broadly neutralizing antibodies (bnAb) against Influenza virus target conserved conformational epitopes of the glycoprotein hemagglutinin (HA), Sun et al. characterize a lineage of bnAbs that neutralize group 1 and 2 strains. Structural characterization shows that antibody 28-12 binds a continuous epitope within H3 (group 2) but requires a conformational epitope for H1 (group 1) binding. Comparison of germline-reverted Ab and intermediate mutants provides evidence for an evolutionary adaptation from group 2 to group 1 strain.","Most structurally characterized broadly neutralizing antibodies (bnAbs) against influenza A viruses (IAVs) target the conserved conformational epitopes of hemagglutinin (HA). Here, we report a lineage of naturally occurring human antibodies sharing the same germline gene, V(H)3-48/V(K)1-12. These antibodies broadly neutralize the major circulating strains of IAV in vitro and in vivo mainly by binding a contiguous epitope of H3N2 HA, but a conformational epitope of H1N1 HA, respectively. Our structural and functional studies of antibody 28-12 revealed that the continuous amino acids in helix A, particularly N49(HA2) of H3 HA, are critical to determine the binding feature with 28-12. In contrast, the conformational epitope feature is dependent on the discontinuous segments involving helix A, the fusion peptide, and several HA1 residues within H1N1 HA. We report that this antibody was initially selected by H3 (group 2) viruses and evolved via somatic hypermutation to enhance the reactivity to H3 and acquire cross-neutralization to H1 (group 1) virus. These findings enrich our understanding of different antigenic determinants of heterosubtypic influenza viruses for the recognition of bnAbs and provide a reference for the design of influenza vaccines and more effective antiviral drugs."]
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收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China["2018YFA0507402","2017YFA0503503"] ; Key International Partnership Program of the Chinese Academy of Sciences[153D31KYSB20180055] ; Strategic Priority Research Program of the Chinese Academy of Sciences["XDB19000000","XDB37040103"] ; National Natural Science Foundation of China[32100751,32100123,32130056]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000789924700004
出版者	NATURE PORTFOLIO
Scopus 记录号	2-s2.0-85129323477
来源库	Scopus
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/180901
专题	生命科学与技术学院_硕士生生命科学与技术学院_PI研究组_王皞鹏组生命科学与技术学院_特聘教授组_孙兵组生命科学与技术学院_博士生
通讯作者	Ling, Zhiyang; Wang, Dayan; Cong, Yao; Sun, Bing
作者单位	1.State Key Laboratory of Cell Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences; University of Chinese Academy of Sciences,Shanghai,200031,China 2.State Key Laboratory of Molecular Biology,National Center for Protein Science Shanghai,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai,200031,China 3.Chinese National Influenza Center,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China 4.Shanghai Public Health Clinical Center,Fudan University,Shanghai,201058,China 5.The National Facility for Protein Science in Shanghai (NFPS),Shanghai,201210,China 6.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China 7.State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China 8.Unit of Herpesvirus and Molecular Virology,Key Laboratory of Molecular Virology & Immunology,Institut Pasteur of Shanghai,Chinese Academy of Sciences,University of the Chinese Academy of Sciences,Shanghai,200031,China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Sun, Xiaoyu,Liu, Caixuan,Lu, Xiao,et al. Unique binding pattern for a lineage of human antibodies with broad reactivity against influenza A virus[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Sun, Xiaoyu.,Liu, Caixuan.,Lu, Xiao.,Ling, Zhiyang.,Yi, Chunyan.,...&Sun, Bing.(2022).Unique binding pattern for a lineage of human antibodies with broad reactivity against influenza A virus.NATURE COMMUNICATIONS,13(1).
MLA	Sun, Xiaoyu,et al."Unique binding pattern for a lineage of human antibodies with broad reactivity against influenza A virus".NATURE COMMUNICATIONS 13.1(2022).