Generation of Piezo1-CreER transgenic mice for visualization and lineage tracing of mechanical force responsive cells in vivo

doi:10.1002/dvg.23476

	Generation of Piezo1-CreER transgenic mice for visualization and lineage tracing of mechanical force responsive cells in vivo
	Li, Xufeng 1; Zhang, Zhenqian 2; Han, Maoying 2; Li, Yang 1; He, Lingjuan 3; Zhou, Bin1,2,4
	2022-05
发表期刊	GENESIS (IF:2.4[JCR-2023],2.1[5-Year])
ISSN	1526-954X
EISSN	1526-968X
发表状态	已发表
DOI	10.1002/dvg.23476
摘要	Cells and tissues are exposed to a wide range of mechanical stimuli during development, tissue homeostasis, repair, and regeneration. Over the past few decades, mechanosensitive ion channels (MSCs), as force-sensing integral membrane proteins, have attracted great attention with regard to their structural dynamics and mechanics at the molecular level and functions in various cells. Piezo-type MSC component 1 (Piezo1) is a newly discovered MSC; it is inherently mechanosensitive. However, which type of cells express Piezo1 in vivo remains unclear. To detect and trace Piezo1-expressing cells, we generated and characterized a novel tamoxifen-inducible Cre knock-in mouse line, Piezo1-CreER, which expresses CreER recombinase under the control of the endogenous Piezo1 promoter. Using this genetic tool, we detected the expression of Piezo1 in various cell types at the embryonic, neonatal, and adult stages. Our data showed that Piezo1 was highly expressed in endothelial cells in all the three stages, while the Piezo1 expression in epithelial cells was dynamic during development and growth. In summary, we established a new genetic tool, Piezo1-CreER, to study Piezo1-expressing cells in vivo during development, injury response, and tissue repair and regeneration.
关键词	genetics mammal organism process tissue
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Key Research & Development Program of China["2021YFA0805100","2019YFA0110403","2019YFA0802000"] ; National Science Foundation of China[82088101,31922032,31730112,91849202,32050087]
WOS研究方向	Developmental Biology ; Genetics & Heredity
WOS类目	Developmental Biology ; Genetics & Heredity
WOS记录号	WOS:000789594800001
出版者	WILEY
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/180884
专题	生命科学与技术学院_特聘教授组_周斌组
通讯作者	He, Lingjuan; Zhou, Bin
作者单位	1.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab C, Shanghai, Peoples R China 3.Westlake Univ, Sch Life Sci, Hangzhou, Zhejiang, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Xufeng,Zhang, Zhenqian,Han, Maoying,et al. Generation of Piezo1-CreER transgenic mice for visualization and lineage tracing of mechanical force responsive cells in vivo[J]. GENESIS,2022.
APA	Li, Xufeng,Zhang, Zhenqian,Han, Maoying,Li, Yang,He, Lingjuan,&Zhou, Bin.(2022).Generation of Piezo1-CreER transgenic mice for visualization and lineage tracing of mechanical force responsive cells in vivo.GENESIS.
MLA	Li, Xufeng,et al."Generation of Piezo1-CreER transgenic mice for visualization and lineage tracing of mechanical force responsive cells in vivo".GENESIS (2022).