G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling

doi:doi.org/ 10.3390/molecules27092658

	G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling
	Ge, Haoyi1,2 ; Wang, Huixia1 ; Pan, Benxun1,2 ; Feng, Dandan1,2 ; Guo, Canyong1,2 ; Yang, Lingyun1 ; Liu, Dongsheng1 ; Wüthrich, Kurt1,3,4
	2022-04
发表期刊	MOLECULES (IF:4.2[JCR-2023],4.6[5-Year])
ISSN	1420-3049
EISSN	1420-3049
卷号	27 期号:9
发表状态	已发表
DOI	doi.org/ 10.3390/molecules27092658
摘要	G protein-coupled receptors (GPCRs) are a large membrane protein family found in higher organisms, including the human body. GPCRs mediate cellular responses to diverse extracellular stimuli and thus control key physiological functions, which makes them important targets for drug design. Signaling by GPCRs is related to the structure and dynamics of these proteins, which are modulated by extrinsic ligands as well as by intracellular binding partners such as G proteins and arrestins. Here, we review some basics of using nuclear magnetic resonance (NMR) spectroscopy in solution for the characterization of GPCR conformations and intermolecular interactions that relate to transmembrane signaling.
关键词	G protein-coupled receptors 19F-NMR membrane mimetics stable-isotope labeling in-membrane chemical modification amino-acid-specific NMR labeling equence-specific NMR labeling F-19-NMR sequence-specific NMR labeling
学科门类	理学::生物学
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31971153,21904088]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000799271400001
出版者	MDPI
原始文献类型	Review
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/180860
专题	生命科学与技术学院_博士生 iHuman研究所_特聘教授组_Kurt Wuthrich组 iHuman研究所_公共科研平台_生命科学NMR平台
通讯作者	Wüthrich, Kurt
作者单位	1.iHuman Institute, ShanghaiTech University 2.School of Life Science and Technology, ShanghaiTech University 3.Department of Integrative Structural and Computational Biology, Scripps Research 4.Institute of Molecular Biology and Biophysics, ETH Zürich
第一作者单位	iHuman研究所; 生命科学与技术学院
通讯作者单位	iHuman研究所
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Ge, Haoyi,Wang, Huixia,Pan, Benxun,et al. G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling[J]. MOLECULES,2022,27(9).
APA	Ge, Haoyi.,Wang, Huixia.,Pan, Benxun.,Feng, Dandan.,Guo, Canyong.,...&Wüthrich, Kurt.(2022).G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling.MOLECULES,27(9).
MLA	Ge, Haoyi,et al."G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling".MOLECULES 27.9(2022).