Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography

doi:10.1002/elps.201600066

	Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography
	Qiu, Danye 1; Li, Feng 1; Zhang, Mingyu 1; Kang, Jingwu1,2
	2016-07
发表期刊	ELECTROPHORESIS (IF:3.0[JCR-2023],2.8[5-Year])
ISSN	0173-0835
卷号	37 期号:12 页码:1725-1732
发表状态	已发表
DOI	10.1002/elps.201600066
摘要	A hydrophilic monolithic CEC column was prepared by thermal copolymerization of zwitterionic monomer 2-methacryloyloxyethyl phosphorylcholine (MPC), pentaerythritol triacrylate (PETA), either methacrylatoethyl trimethyl ammonium chloride (META) or sodium 2-methylpropene-1-sulfonate (MPS) in a polar binary porogen consisting of methanol and THF. A typical hydrophilic interaction LC retention mechanism was observed for low-molecular weight polar compounds including amides, nucleotides, and nucleosides in the separation mode of hydrophilic interaction CEC, when high content of ACN (>60%) was used as the mobile phase. The effect of the electrostatic interaction between the analytes and the stationary phase was found to be negligible. The poly(MPCco- PETA-co-META or MPS) monolithic columns have an average column efficiency of 40 000 plates/m and displayed with a satisfactory repeatability in terms of migration time and peak areas. Finally, the column was successfully applied to determine the impurities of a positively charged drug pramipexole which are often separated by ion pair RP chromatography due to their high hydrophilicity. All four components can be baseline separated within 5 min with BGE consisting of ACN/20 mM ammonium formate buffer (pH 3.0;80/20).
关键词	Hydrophilic interaction capillary electrochromatography Impurity analysis Pramipexole Zwitterionic monolithic column
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000379132300015
出版者	WILEY
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; ZWITTERIONIC STATIONARY-PHASE ; RIGID POLYMER MONOLITHS ; SEPARATION MEDIA ; HUMAN PLASMA ; ORGANIC MONOLITHS ; POROUS PROPERTIES ; POLAR ANALYTES ; MIXED-MODE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1790
专题	物质科学与技术学院_特聘教授组_康经武组
通讯作者	Kang, Jingwu
作者单位	1.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Lingling Rd 345, Shanghai 200032, Peoples R China 2.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Qiu, Danye,Li, Feng,Zhang, Mingyu,et al. Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography[J]. ELECTROPHORESIS,2016,37(12):1725-1732.
APA	Qiu, Danye,Li, Feng,Zhang, Mingyu,&Kang, Jingwu.(2016).Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography.ELECTROPHORESIS,37(12),1725-1732.
MLA	Qiu, Danye,et al."Preparation of phosphorylcholine-based hydrophilic monolithic column and application for analysis of drug-related impurities with capillary electrochromatography".ELECTROPHORESIS 37.12(2016):1725-1732.