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Enhancement of prime editing via xrRNA motif-joined pegRNA | |
Zhang, Guiquan1; Liu, Yao2; Huang, Shisheng3; Qu, Shiyuan3; Cheng, Daolin1; Yao, Yuan4; Ji, Quanjiang5; Wang, Xiaolong2; Huang, Xingxu3,6; Liu, Jianghuai1 | |
2022-04-06 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
EISSN | 2041-1723 |
卷号 | 13期号:1 |
发表状态 | 已发表 |
DOI | 10.1038/s41467-022-29507-x |
摘要 | The prime editors (PEs) have shown great promise for precise genome modification. However, their suboptimal efficiencies present a significant technical challenge. Here, by appending a viral exoribonuclease-resistant RNA motif (xrRNA) to the 3′-extended portion of pegRNAs for their increased resistance against degradation, we develop an upgraded PE platform (xrPE) with substantially enhanced editing efficiencies in multiple cell lines. A pan-target average enhancement of up to 3.1-, 4.5- and 2.5-fold in given cell types is observed for base conversions, small deletions, and small insertions, respectively. Additionally, xrPE exhibits comparable edit:indel ratios and similarly minimal off-target editing as the canonical PE3. Of note, parallel comparison of xrPE to the most recently developed epegRNA-based PE system shows their largely equivalent editing performances. Our study establishes a highly adaptable platform of improved PE that shall have broad implications. |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
Scopus 记录号 | 2-s2.0-85127716166 |
来源库 | Scopus |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/176059 |
专题 | 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_黄行许组 物质科学与技术学院_PI研究组_季泉江组 |
通讯作者 | Wang, Xiaolong; Huang, Xingxu; Liu, Jianghuai |
作者单位 | 1.State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study,Model Animal Research Center at Medical School of Nanjing University,Nanjing,210061,China 2.Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,712100,China 3.Gene Editing Center,School of Life Science and Technology,ShanghaiTech University,Shanghai,100 Haike Rd., Pudong New Area,201210,China 4.Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University,Hangzhou,311215,China 5.School of Physical Science and Technology,ShanghaiTech University,Shanghai,100 Haike Rd., Pudong New Area,201210,China 6.Zhejiang Laboratory,Hangzhou,311100,China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Zhang, Guiquan,Liu, Yao,Huang, Shisheng,et al. Enhancement of prime editing via xrRNA motif-joined pegRNA[J]. NATURE COMMUNICATIONS,2022,13(1). |
APA | Zhang, Guiquan.,Liu, Yao.,Huang, Shisheng.,Qu, Shiyuan.,Cheng, Daolin.,...&Liu, Jianghuai.(2022).Enhancement of prime editing via xrRNA motif-joined pegRNA.NATURE COMMUNICATIONS,13(1). |
MLA | Zhang, Guiquan,et al."Enhancement of prime editing via xrRNA motif-joined pegRNA".NATURE COMMUNICATIONS 13.1(2022). |
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