Enhancement of prime editing via xrRNA motif-joined pegRNA
Zhang, Guiquan1; Liu, Yao2; Huang, Shisheng3; Qu, Shiyuan3; Cheng, Daolin1; Yao, Yuan4; Ji, Quanjiang5; Wang, Xiaolong2; Huang, Xingxu3,6; Liu, Jianghuai1
2022-04-06
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
EISSN2041-1723
卷号13期号:1
发表状态已发表
DOI10.1038/s41467-022-29507-x
摘要

The prime editors (PEs) have shown great promise for precise genome modification. However, their suboptimal efficiencies present a significant technical challenge. Here, by appending a viral exoribonuclease-resistant RNA motif (xrRNA) to the 3′-extended portion of pegRNAs for their increased resistance against degradation, we develop an upgraded PE platform (xrPE) with substantially enhanced editing efficiencies in multiple cell lines. A pan-target average enhancement of up to 3.1-, 4.5- and 2.5-fold in given cell types is observed for base conversions, small deletions, and small insertions, respectively. Additionally, xrPE exhibits comparable edit:indel ratios and similarly minimal off-target editing as the canonical PE3. Of note, parallel comparison of xrPE to the most recently developed epegRNA-based PE system shows their largely equivalent editing performances. Our study establishes a highly adaptable platform of improved PE that shall have broad implications.

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收录类别SCI ; SCIE
语种英语
Scopus 记录号2-s2.0-85127716166
来源库Scopus
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/176059
专题生命科学与技术学院_博士生
生命科学与技术学院_PI研究组_黄行许组
物质科学与技术学院_PI研究组_季泉江组
通讯作者Wang, Xiaolong; Huang, Xingxu; Liu, Jianghuai
作者单位1.State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study,Model Animal Research Center at Medical School of Nanjing University,Nanjing,210061,China
2.Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,712100,China
3.Gene Editing Center,School of Life Science and Technology,ShanghaiTech University,Shanghai,100 Haike Rd., Pudong New Area,201210,China
4.Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University,Hangzhou,311215,China
5.School of Physical Science and Technology,ShanghaiTech University,Shanghai,100 Haike Rd., Pudong New Area,201210,China
6.Zhejiang Laboratory,Hangzhou,311100,China
通讯作者单位生命科学与技术学院
推荐引用方式
GB/T 7714
Zhang, Guiquan,Liu, Yao,Huang, Shisheng,et al. Enhancement of prime editing via xrRNA motif-joined pegRNA[J]. NATURE COMMUNICATIONS,2022,13(1).
APA Zhang, Guiquan.,Liu, Yao.,Huang, Shisheng.,Qu, Shiyuan.,Cheng, Daolin.,...&Liu, Jianghuai.(2022).Enhancement of prime editing via xrRNA motif-joined pegRNA.NATURE COMMUNICATIONS,13(1).
MLA Zhang, Guiquan,et al."Enhancement of prime editing via xrRNA motif-joined pegRNA".NATURE COMMUNICATIONS 13.1(2022).
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