ShanghaiTech University Knowledge Management System
AAV-ie-K558R mediated cochlear gene therapy and hair cell regeneration | |
2022-04-22 | |
发表期刊 | SIGNAL TRANSDUCTION AND TARGETED THERAPY (IF:40.8[JCR-2023],40.5[5-Year]) |
ISSN | 2095-9907 |
EISSN | 2059-3635 |
卷号 | 7期号:1 |
发表状态 | 已发表 |
DOI | 10.1038/s41392-022-00938-8 |
摘要 | The cochlea consists of multiple types of cells, including hair cells, supporting cells and spiral ganglion neurons, and is responsible for converting mechanical forces into electric signals that enable hearing. Genetic and environmental factors can result in dysfunctions of cochlear and auditory systems. In recent years, gene therapy has emerged as a promising treatment in animal deafness models. One major challenge of the gene therapy for deafness is to effectively deliver genes to specific cells of cochleae. Here, we screened and identified an AAV-ie mutant, AAV-ie-K558R, that transduces hair cells and supporting cells in the cochleae of neonatal mice with high efficiency. AAV-ie-K558R is a safe vector with no obvious deficits in the hearing system. We found that AAV-ie-K558R can partially restore the hearing loss in Prestin KO mice and, importantly, deliver Atoh1 into cochlear supporting cells to generate hair cell-like cells. Our results demonstrate the clinical potential of AAV-ie-K558R for treating the hearing loss caused by hair cell death. |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
资助项目 | Key Project of the National Natural Science Foundation of China[NSFC 81730028] ; National Key Technology Research and Development Program of the Ministry of Science and Technology of China[2017YFC1001800] ; Shanghai Key Laboratory of Translational Medicine on Ear and Nose diseases[14DZ2260300] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX05] ; National Natural Science Foundation of China[31771130,82122019] |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
WOS记录号 | WOS:000784713200001 |
出版者 | SPRINGERNATURE |
Scopus 记录号 | 2-s2.0-85128669313 |
来源库 | Scopus |
引用统计 | 正在获取...
|
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/176041 |
专题 | 生命科学与技术学院_硕士生 生命科学与技术学院_PI研究组_林照博组 iHuman研究所_PI研究组_钟桂生组 生命科学与技术学院_博士生 |
通讯作者 | Chai, Renjie; Zhong, Guisheng; Wu, Hao |
作者单位 | 1.Department of Otolaryngology-Head and Neck Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China 2.Ear Institute,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China 3.Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases,Shanghai,200011,China 4.iHuman Institute,ShanghaiTech University,Shanghai,201210,China 5.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China 6.State Key Laboratory of Bioelectronics,Department of Otolaryngology Head and Neck Surgery,Zhongda Hospital,School of Life Sciences and Technology,Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research,Southeast University,Nanjing,210096,China 7.Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,226001,China 8.Institute for Stem Cell and Regeneration,Chinese Academy of Science,Beijing,China 9.Beijing Key Laboratory of Neural Regeneration and Repair,Capital Medical University,Beijing,100069,China |
通讯作者单位 | iHuman研究所; 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Tao, Yong,Liu, Xiaoyi,Yang, Liu,et al. AAV-ie-K558R mediated cochlear gene therapy and hair cell regeneration[J]. SIGNAL TRANSDUCTION AND TARGETED THERAPY,2022,7(1). |
APA | Tao, Yong.,Liu, Xiaoyi.,Yang, Liu.,Chu, Cenfeng.,Tan, Fangzhi.,...&Wu, Hao.(2022).AAV-ie-K558R mediated cochlear gene therapy and hair cell regeneration.SIGNAL TRANSDUCTION AND TARGETED THERAPY,7(1). |
MLA | Tao, Yong,et al."AAV-ie-K558R mediated cochlear gene therapy and hair cell regeneration".SIGNAL TRANSDUCTION AND TARGETED THERAPY 7.1(2022). |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 |
个性服务 |
查看访问统计 |
谷歌学术 |
谷歌学术中相似的文章 |
[Tao, Yong]的文章 |
[Liu, Xiaoyi]的文章 |
[Yang, Liu]的文章 |
百度学术 |
百度学术中相似的文章 |
[Tao, Yong]的文章 |
[Liu, Xiaoyi]的文章 |
[Yang, Liu]的文章 |
必应学术 |
必应学术中相似的文章 |
[Tao, Yong]的文章 |
[Liu, Xiaoyi]的文章 |
[Yang, Liu]的文章 |
相关权益政策 |
暂无数据 |
收藏/分享 |
修改评论
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。