Zcchc12-Containing Nociceptors Are Required for Noxious Heat Sensation

doi:10.1523/JNEUROSCI.1427-21.2022

	Zcchc12-Containing Nociceptors Are Required for Noxious Heat Sensation
	Wu, Dan 1,2; Chen, Yan 3,4,5,6; Li, Zhen 4,5,6; Xie, Hong 4,5,6; Wang, Sashuang 7,8; Lu, Yingjin 3,4,5,6,9; Bao, Lan10,11 ; Zhang, Xu1,2,3,4,5,6,10 ; Li, Changlin 3,4,5,6,9
	2022-03-30
发表期刊	JOURNAL OF NEUROSCIENCE (IF:4.4[JCR-2023],5.3[5-Year])
ISSN	0270-6474
EISSN	1529-2401
卷号	42 期号:13
发表状态	已发表
DOI	10.1523/JNEUROSCI.1427-21.2022
摘要	DRG neurons are classified into distinct types to mediate the somatosensation with different modalities. Recently, transcriptional profilings of DRG neurons by single-cell RNA-sequencing have provided new insights into the neuron typing and functional properties. Zinc-finger CCHC domain-containing 12 (Zcchc12) was reported to be the representative marker for a subtype of galanin-positive (Gal(+)) DRG neurons. However, the characteristics and functions of Zcchc12(+) neurons are largely unknown. Here, we genetically labeled Zcchc12(+) neurons in Zcchc12-CreERT2::Ai9 mice, and verified that Zcchc12 represented a new subpopulation of DRG neurons in both sexes. Zcchc12(+) neurons centrally innervated the superficial laminae in spinal dorsal horn, and peripherally terminated as free nerve endings in the epidermis and cluster-shaped fibers in the dermis of footpads and nearby. In addition, Zcchc12(+) neurons also formed circumferential endings surrounding the hair follicles in hairy skin. Functionally, in vivo calcium imaging in DRGs revealed that Zcchc12(+) neurons were polymodal nociceptors and could be activated by mechanical and noxious thermal stimuli. Behavioral tests showed that selective ablation of Zcchc12(+) DRG neurons reduced the sensitivity to noxious heat in mice. Together, we identified a new subpopulation of Zcchc12(+) nociceptors essential for noxious heat sensation.
关键词	heat nociception mechanoheat nociceptor Zcchc12
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31671094,32071001,31630033,31600853] ; Chinese Academy of Sciences[QYZDYSSW-SMC007] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB39050100] ; Science and Technology Commission of Shanghai Municipality[18JC1420301] ; Innovation Fund for Medical Sciences of Chinese Academy of Medical Sciences[2019-I2M-5-082]
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000779638100007
出版者	SOC NEUROSCIENCE
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/176018
专题	生命科学与技术学院_特聘教授组_张旭组生命科学与技术学院_特聘教授组_鲍岚组
通讯作者	Zhang, Xu; Li, Changlin
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, State Key Lab Neurosci, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guangdong Inst Intelligence Sci & Technol, Hengqin 519031, Zhuhai, Peoples R China 4.Chinese Acad Med Sci, Res Unit Pain Med, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Shanghai Adv Res Inst, Inst Brain Intelligence Technol, SIMR Joint Lab Drug Innovat, Shanghai 201210, Peoples R China 6.Shanghai Res Ctr Brain Sci & Brain Inspired Intel, Shanghai 201210, Peoples R China 7.Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Dept Pain Med, Shenzhen 518052, Peoples R China 8.Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Shenzhen Municipal Key Lab Pain Med, Shenzhen 518052, Peoples R China 9.Xuhui Cent Hosp, Shanghai 200031, Peoples R China 10.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 11.Chinese Acad Sci, Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wu, Dan,Chen, Yan,Li, Zhen,et al. Zcchc12-Containing Nociceptors Are Required for Noxious Heat Sensation[J]. JOURNAL OF NEUROSCIENCE,2022,42(13).
APA	Wu, Dan.,Chen, Yan.,Li, Zhen.,Xie, Hong.,Wang, Sashuang.,...&Li, Changlin.(2022).Zcchc12-Containing Nociceptors Are Required for Noxious Heat Sensation.JOURNAL OF NEUROSCIENCE,42(13).
MLA	Wu, Dan,et al."Zcchc12-Containing Nociceptors Are Required for Noxious Heat Sensation".JOURNAL OF NEUROSCIENCE 42.13(2022).