PUMILIO proteins promote colorectal cancer growth via suppressing p21

doi:10.1038/s41467-022-29309-1

	PUMILIO proteins promote colorectal cancer growth via suppressing p21
	Gong, Yuanyuan1,2,3 ; Liu, Zukai1 ; Yuan, Yihang 4; Yang, Zhenzhen1 ; Zhang, Jiawei1 ; Lu, Qin 4; Wang, Wei1 ; Fang, Chao 4,5; Lin, Haifan 6; Liu, Sanhong1,7
	2022-03-25
发表期刊	NATURE COMMUNICATIONS
EISSN	2041-1723
卷号	13 期号:1
发表状态	已发表
DOI	10.1038/s41467-022-29309-1
摘要	PUMILIO (PUM) proteins belong to the highly conserved PUF family post-transcriptional regulators involved in diverse biological processes. However, their function in carcinogenesis remains under-explored. Here, we report that Pum1 and Pum2 display increased expression in human colorectal cancer (CRC). Intestine-specific knockout of Pum1 and Pum2 in mice significantly inhibits the progression of colitis-associated cancer in the AOM/DSS model. Knockout or knockdown of Pum1 and/or Pum2 in human CRC cells result in a significant decrease in the tumorigenicity and delayed G1/S transition. We identify p21/Cdkn1a as a direct target of PUM1. Abrogation of the PUM1 binding site in the p21 mRNA also results in decreased cancer cell growth and delayed G1/S transition. Furthermore, intravenous injection of nanoparticle-encapsulated anti-Pum1 and Pum2 siRNAs reduces colorectal tumor growth in murine orthotopic colon cancer models. These findings reveal the requirement of PUM proteins for CRC progression and their potential as therapeutic targets.
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收录类别	SCIE
语种	英语
Scopus 记录号	2-s2.0-85127265977
来源库	Scopus
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/169263
专题	免疫化学研究所_特聘教授组_干细胞生物学实验室生命科学与技术学院_硕士生免疫化学研究所_公共科研平台_生物医学大数据平台免疫化学研究所_特聘教授组_Erez Lieberman Aiden组
通讯作者	Fang, Chao; Lin, Haifan; Liu, Sanhong
作者单位	1.Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology,ShanghaiTech University,Shanghai,China 2.Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai,China 3.University of Chinese Academy of Sciences,Beijing,China 4.Hongqiao International Institute of Medicine,Tongren Hospital and State Key Laboratory of Oncogenes and Related Genes,Department of Pharmacology and Chemical Biology,Shanghai Jiao Tong University School of Medicine (SJTU-SM),Shanghai,China 5.Key Laboratory of Basic Pharmacology of Ministry of Education & Joint International Research Laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi,China 6.The Yale Stem Cell Center and Department of Cell Biology,Yale University School of Medicine,New Haven,United States 7.Shanghai Frontiers Science Center of TCM Chemical Biology,Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai,China
第一作者单位	免疫化学研究所
通讯作者单位	免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Gong, Yuanyuan,Liu, Zukai,Yuan, Yihang,et al. PUMILIO proteins promote colorectal cancer growth via suppressing p21[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Gong, Yuanyuan.,Liu, Zukai.,Yuan, Yihang.,Yang, Zhenzhen.,Zhang, Jiawei.,...&Liu, Sanhong.(2022).PUMILIO proteins promote colorectal cancer growth via suppressing p21.NATURE COMMUNICATIONS,13(1).
MLA	Gong, Yuanyuan,et al."PUMILIO proteins promote colorectal cancer growth via suppressing p21".NATURE COMMUNICATIONS 13.1(2022).