Global profiling of regulatory elements in the histone benzoylation pathway

doi:10.1038/s41467-022-29057-2

	Global profiling of regulatory elements in the histone benzoylation pathway
	Wang, Duo 1,2; Yan, Fuxiang 1,2; Wu, Ping 3; Ge, Kexue 1,2; Li, Muchun1,2,4 ; Li, Tingting 1,2; Gao, Ying 1; Peng, Chao 3; Chen, Yong1,2,4
	2022-03-16
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	13 期号:1
发表状态	已发表
DOI	10.1038/s41467-022-29057-2
摘要	Lysine benzoylation (Kbz) is a recently discovered post-translational modification associated with active transcription. However, the proteins for maintaining and interpreting Kbz and the physiological roles of Kbz remain elusive. Here, we systematically characterize writer, eraser, and reader proteins of histone Kbz in S. cerevisiae using proteomic, biochemical, and structural approaches. Our study identifies 27 Kbz sites on yeast histones that can be regulated by cellular metabolic states. The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex and NAD-dependent histone deacetylase Hst2 could function as the writer and eraser of histone Kbz, respectively. Crystal structures of Hst2 complexes reveal the molecular basis for Kbz recognition and catalysis by Hst2. In addition, we demonstrate that a subset of YEATS domains and bromodomains serve as Kbz readers, and structural analyses reveal how YEATS and bromodomains recognize Kbz marks. Moreover, the proteome-wide screening of Kbz-modified proteins identifies 207 Kbz sites on 149 non-histone proteins enriched in ribosome biogenesis, glycolysis/gluconeogenesis, and rRNA processing pathways. Our studies identify regulatory elements for the Kbz pathway and provide a framework for dissecting the biological functions of lysine benzoylation.
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收录类别	SCIE
语种	英语
Scopus 记录号	2-s2.0-85126331619
来源库	Scopus
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/165034
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_陈勇组
通讯作者	Peng, Chao; Chen, Yong
作者单位	1.State Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai,200031,China 2.University of Chinese Academy of Sciences,Beijing,100049,China 3.National Facility for Protein Science in Shanghai,Zhangjiang Lab,Shanghai Advanced Research Institute,Chinese Academy of Science,Shanghai,201210,China 4.School of Life Science and Technology,ShanghaiTech University,Shanghai,201210,China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Wang, Duo,Yan, Fuxiang,Wu, Ping,et al. Global profiling of regulatory elements in the histone benzoylation pathway[J]. NATURE COMMUNICATIONS,2022,13(1).
APA	Wang, Duo.,Yan, Fuxiang.,Wu, Ping.,Ge, Kexue.,Li, Muchun.,...&Chen, Yong.(2022).Global profiling of regulatory elements in the histone benzoylation pathway.NATURE COMMUNICATIONS,13(1).
MLA	Wang, Duo,et al."Global profiling of regulatory elements in the histone benzoylation pathway".NATURE COMMUNICATIONS 13.1(2022).