Nde1 is a Rab9 effector for loading late endosomes to cytoplasmic dynein motor complex

doi:10.1016/j.str.2021.10.013

	Nde1 is a Rab9 effector for loading late endosomes to cytoplasmic dynein motor complex
	Zhang, Yifan 1; Chen, Ziyue 1; Wang, Fang 1; Sun, Honghua 1; Zhu, Xueliang1,2,3 ; Ding, Jianping1,2,3 ; Zhang, Tianlong 1,4
	2022-03-03
发表期刊	STRUCTURE (IF:4.4[JCR-2023],4.3[5-Year])
ISSN	0969-2126
EISSN	1878-4186
卷号	30 期号:3
发表状态	已发表
DOI	10.1016/j.str.2021.10.013
摘要	Rab9 is mainly located on late endosomes and required for their intracellular transport to trans-Golgi network (TGN). The cytoplasmic dynein motor, together with its regulatory proteins Nde1/Ndel1 and Lis1, controls intracellular retrograde transport of membranous organelles along the microtubule network. How late endosomes are tethered to the microtubule-based motor dynein for their retrograde transport remains unclear. Here, we demonstrate that the guanosine triphosphate (GTP)-bound Rab9A/B specifically uses Nde1/Ndel1 as an effector to interact with the dynein motor complex. We determined the crystal structure of Rab9A-GTP in complex with the Rab9-binding region of Nde1. The functional roles of key residues involved in the Rab9A-Nde1 interaction are verified using biochemical and cell biology assays. Rab9A mutants unable to bind to Nde1 also failed to associate with dynein, Lis1, and dynactin. Therefore, Nde1 is a Rab9 effector that tethers Rab9-associated late endosomes to the dynein motor for their retrograde transport to the TGN.
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31530013,31870722] ; National Key Research and Development Program of China["2020YFA0509000","2017YFA0503500"] ; Chinese Academy of Sciences[XDB37030305]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000766494300009
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/165009
专题	生命科学与技术学院_特聘教授组_朱学良组生命科学与技术学院_特聘教授组_丁建平组
通讯作者	Zhu, Xueliang; Ding, Jianping; Zhang, Tianlong
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, 1 Xiangshan Rd, Hangzhou 310024, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Hua Xia Zhong Rd, Shanghai 201210, Peoples R China 4.Shanghai Univ, Affiliated Nantong Hosp, Peoples Hosp Nantong 6, Inst Geriatr,Shanghai Engn Res Ctr Organ Repair,S, 500 Yonghe Rd, Nantong 226011, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Yifan,Chen, Ziyue,Wang, Fang,et al. Nde1 is a Rab9 effector for loading late endosomes to cytoplasmic dynein motor complex[J]. STRUCTURE,2022,30(3).
APA	Zhang, Yifan.,Chen, Ziyue.,Wang, Fang.,Sun, Honghua.,Zhu, Xueliang.,...&Zhang, Tianlong.(2022).Nde1 is a Rab9 effector for loading late endosomes to cytoplasmic dynein motor complex.STRUCTURE,30(3).
MLA	Zhang, Yifan,et al."Nde1 is a Rab9 effector for loading late endosomes to cytoplasmic dynein motor complex".STRUCTURE 30.3(2022).