Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins

doi:10.1016/j.molcel.2016.10.007

	Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins
	Wu, Huang 1,3; Yin, Qing-Fei 1; Luo, Zheng 2,3; Yao, Run-Wen 1,3; Zheng, Chuan-Chuan 1,3; Zhang, Jun 1; Xiang, Jian-Feng 1,2; Yang, Li2,3,4 ; Chen, Ling-Ling1,3,4
	2016-11-03
发表期刊	MOLECULAR CELL (IF:14.5[JCR-2023],16.6[5-Year])
ISSN	1097-2765
卷号	64 期号:3 页码:534-548
发表状态	已发表
DOI	10.1016/j.molcel.2016.10.007
摘要	We identify a type of polycistronic transcript-derived long noncoding RNAs (lncRNAs) that are 50 small nucleolar RNA (snoRNA) capped and 30 polyadenylated (SPAs). SPA processing is associated with nascent mRNA 30 processing and kinetic competition between XRN2 trimming and Pol II elongation. Following cleavage/polyadenylation of its upstream gene, the downstream uncapped pre-SPA is trimmed by XRN2 until this exonuclease reaches the co-transcriptionally assembled snoRNP. This snoRNP complex prevents further degradation, generates a snoRNA 50 end, and allows continuous Pol II elongation. The imprinted 15q11-q13 encodes two SPAs that are deleted in Prader-Willi syndrome (PWS) patients. These lncRNAs form a nuclear accumulation that is enriched in RNA binding proteins (RBPs) including TDP43, RBFOX2, and hnRNP M. Generation of a human PWS cellular model by depleting these lncRNAs results in altered patterns of RBPs binding and alternative splicing. Together, these results expand the diversity of lncRNAs and provide additional insights into PWS pathogenesis.
收录类别	SCI
语种	英语
资助项目	NSFC[91440202] ; NSFC[31471241] ; NSFC[31322018] ; NSFC[31271376]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000389515300012
出版者	CELL PRESS
WOS关键词	PRADER-WILLI-SYNDROME ; PRE-MESSENGER-RNA ; SMALL NUCLEOLAR RNAS ; LONG NONCODING RNAS ; ELONGATION RATE ; STEM-CELLS ; TRANSCRIPT ; GENE ; DELETION ; REVEALS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1647
专题	生命科学与技术学院_特聘教授组_杨力组生命科学与技术学院_特聘教授组_陈玲玲组
通讯作者	Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, Shanghai Key Lab Mol Androl, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab M, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci,Key Lab Computat Biol, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200031, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci, Shanghai 200031, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Wu, Huang,Yin, Qing-Fei,Luo, Zheng,et al. Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins[J]. MOLECULAR CELL,2016,64(3):534-548.
APA	Wu, Huang.,Yin, Qing-Fei.,Luo, Zheng.,Yao, Run-Wen.,Zheng, Chuan-Chuan.,...&Chen, Ling-Ling.(2016).Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins.MOLECULAR CELL,64(3),534-548.
MLA	Wu, Huang,et al."Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins".MOLECULAR CELL 64.3(2016):534-548.